Earthworms and arbuscular mycorrhizal fungi (AMF) have substantial individual effects on the supply and absorption of soil nutrients in terrestrial ecosystems. How their interactions regulate the soil living microbial community and microbial necromass accumulation remains unknown. We hypothesized that the effect of their interaction on the microbial community and necromass accumulation may relate to their controls on status of soil N and P. We conducted a greenhouse experiment manipulating the AMF Rhizophagus intraradices and the earthworm Pontoscolex corethrurus in microcosms planted with a fern Dicranopteris dichotoma. Phospholipid fatty acids and amino sugars were used to indicate the living microbial community and necromass accumulation, respectively. The AMF consistently reduced the biomass of bacterial groups and the concentration of total amino sugars, glucosamine and muramic acid, in the absence of earthworms, but had no effect in their presence. The earthworms increased the concentration of soil total dissolved N, water-soluble N and water-soluble P, and the activity of β–1,4–N-acetylglucosaminidase and phosphatase, and the amount of P in total plant biomass. Redundancy analysis showed that the bacterial biomass was positively correlated with most soil labile N indices and negatively correlated with the amount of N in total plant biomass. Pearson correlation revealed that the bacterial nutritional stress indicator Sat/Mono was negatively correlated with soil NH₄⁺ concentration and positively correlated with the amount of N in total plant biomass. The results suggested that earthworms might negate the suppression effect of AMF on bacterial abundance and necromass accumulation by alleviating AMF-induced N deficiency.

and和丛枝菌根真菌（AMF）对陆地生态系统中土壤养分的供应和吸收具有重大的个体影响。它们之间的相互作用如何调节土壤中的微生物群落和微生物坏死物质的积累仍然未知。我们假设，他们的相互作用对微生物群落和necromass累积效果可能涉及其控制对土壤N和P状态我们进行了一项实验温室操纵AMF Rhizophagus intraradices和蚯蚓Pontoscolex围海蚓在微观种植了羊齿芒双歧瘤。磷脂脂肪酸和氨基糖分别用于指示活的微生物群落和坏死物质的积累。在没有absence的情况下，AMF会不断减少细菌类的生物量以及总氨基糖，氨基葡萄糖和山酸的浓度，但在它们存在的情况下则没有任何作用。increased增加了土壤总溶解氮，水溶性氮和水溶性磷的浓度，并增加了β–1,4– N的活性-乙酰氨基葡糖苷酶和磷酸酶，以及植物总生物量中磷的含量。冗余分析表明，细菌生物量与大多数土壤不稳定的氮指数呈正相关，与植物总生物量中氮的含量呈负相关。皮尔逊相关性表明，细菌营养胁迫指标Sat / Mono与土壤NH ₄ ⁺浓度呈负相关，与植物总生物量中N的含量呈正相关。结果表明earth可能通过缓解AMF引起的氮缺乏而抵消AMF对细菌丰度和坏死积累的抑制作用。