Recent evidence shows that long non-coding RNAs (lncRNAs), a class of non-coding RNAs, are involved in the regulation of reproductive processes. In this study, we identified a lncRNA, TCONS_00814106, that was upregulated in high-fecundity sow ovarian tissues and influenced by reproductive hormones. Bioinformatics analyses and luciferase reporter assays showed that TCONS_00814106 is a miR-1343 target. Cell counting kit (CCK)-8 and apoptosis assays showed that TCONS_00814106 promotes proliferation and inhibits apoptosis in porcine granulosa cells (GCs), and that this could be reversed by miR-1343. Also, we observed that transforming growth factor-β receptor type I (TGFBR1) is a functional target of miR-1343 in GCs. TCONS_00814106 serves as a competing endogenous RNA to regulate TGFBR1 expression by sponging miR-1343, thereby exerting regulatory functions in GCs. Overall, these results provide new insights into the biological function of the lncRNA TCONS_00814106.

最近的证据表明，长链非编码 RNA (lncRNA) 是一类非编码 RNA，参与生殖过程的调节。在本研究中，我们鉴定了一个 lncRNA，TCONS_00814106，它在高繁殖力母猪卵巢组织中上调并受生殖激素的影响。生物信息学分析和荧光素酶报告基因检测表明 TCONS_00814106 是 miR-1343 的靶标。细胞计数试剂盒 (CCK)-8 和细胞凋亡试验表明，TCONS_00814106 促进猪颗粒细胞 (GC) 的增殖并抑制细胞凋亡，并且这可以被 miR-1343 逆转。此外，我们观察到转化生长因子-β 受体 I 型（TGFBR1）是 GC 中 miR-1343 的功能靶点。TCONS_00814106 作为竞争性内源性 RNA 来调节TGFBR1通过海绵 miR-1343 表达，从而在 GC 中发挥调节功能。总体而言，这些结果为 lncRNA TCONS_00814106 的生物学功能提供了新的见解。