Background

The aim of this study is to address the role of HPV in prostate cancer (PCa) development through the inducement of resistance to anoikis.

Methods

In this case-control study, prostate tissues and blood samples were collected from 116 individuals, including 72 cases with PCa and 44 non‐malignant prostate tissue samples as a control group. The expression level of HPV genes (E2, E6, and E7) and cellular genes including anti-apoptotic mediators (Bcl-2 and survivin), tumor suppressor proteins (Rb and p53), and some mediators involved in anoikis resistance and invasiveness (E-cadherin, N-cadherin, Twist, PTPN13 and SLUG) were evaluated.

Results

HPV genome was identified in 36.1% cases and 15.9% control samples, additionally there was found to be a statistic significant association between the presence of HPV and PCa (OR = 1.64, 95% C.I = 0.8–1.8, P-value = 0.023). HPV genotype 16 and 18 were the most prevalent genotype in both in the PCa group and the control group. The expression level of the tumor suppressor proteins (Rb and p53) and anti-apoptotic mediators (Bcl-2 and Survivin) were significantly decreased and increased, respectively, in the HPV-positive specimens compared to the HPV-negative specimens. Furthermore, the mean expression level of N-cadherin, SLUG, and TWIST in the HPV-positive specimens was higher than HPV-negative specimens while the mean expression level of PTPN-13 and E-cadherin genes in the HPV-positive specimens was lower than HPV-negative specimens.

Conclusion

Our study suggests that HPV infection may be involved in the development of PCa metastases by modulating anoikis resistance related genes.

中文翻译：

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人乳头瘤病毒和前列腺癌：病毒表达蛋白在抑制失巢凋亡和诱导转移中的作用

背景

本研究的目的是通过诱导失巢凋亡抗性来解决 HPV 在前列腺癌 (PCa) 发展中的作用。

方法

在本病例对照研究中，采集了 116 名个体的前列腺组织和血液样本，其中 72 例 PCa 患者和 44 例非恶性前列腺组织样本作为对照组。HPV 基因（E2、E6 和 E7）和细胞基因的表达水平，包括抗凋亡介质（Bcl-2 和存活蛋白）、肿瘤抑制蛋白（Rb 和 p53）以及一些参与失巢凋亡抗性和侵袭性的介质（E -钙粘蛋白、N-钙粘蛋白、Twist、PTPN13 和 SLUG）进行了评估。

结果

在 36.1% 的病例和 15.9% 的对照样本中鉴定出 HPV 基因组，此外还发现 HPV 的存在与前列腺癌之间存在统计学上的显着关联（OR = 1.64，95% CI = 0.8–1.8，P 值 = 0.023） . HPV 基因型 16 和 18 是 PCa 组和对照组中最普遍的基因型。与 HPV 阴性样本相比，HPV 阳性样本中肿瘤抑制蛋白（Rb 和 p53）和抗凋亡介质（Bcl-2 和 Survivin）的表达水平分别显着降低和升高。此外，HPV 阳性标本中 N-cadherin、SLUG 和 TWIST 的平均表达水平高于 HPV 阴性标本，而 PTPN-13 和 E-cadherin 基因在 HPV 阳性标本中的平均表达水平较低高于 HPV 阴性标本。

结论

我们的研究表明，HPV 感染可能通过调节失巢凋亡抗性相关基因参与 PCa 转移的发展。