The aim of this study was to investigate the surface topography of remineralized enamel induced by poly(amido amine) (PAMAM) dendrimers and evaluate Streptococcus mutans (S. mutans) adhesion on regenerated enamel for the first time. PAMAM−COOH and PAMAM-NH₂ were used as organic templates to induce enamel surface remineralization. The mineral deposits after remineralization were characterized by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and X-ray diffraction (XRD). The surface topography of the remineralized enamel was analyzed by atomic force microscopy (AFM). An AFM tipless cantilever was functionalized with S. mutans and acted as a force probe to measure the adhesion force between bacteria and the remineralized enamel surface. Colony-forming unit (CFU) counts of biofilm on remineralized enamel surface were performed after 24 h incubation in S. mutans suspension. Both PAMAM−COOH and PAMAM-NH₂ achieved effective remineralization on demineralized enamel surfaces, which smoothed the enamel surface and reduced S. mutans adhesion. PAMAM dendrimers are promising materials for early caries treatment because of their excellent remineralization ability. The remineralization induced by PAMAM dendrimers smoothed the surface and reduced S. mutans adhesion, which could prevent secondary caries.

这项研究的目的是调查由聚（酰胺胺）（PAMAM）树枝状大分子诱导的再矿化釉质的表面形貌，并首次评估变形链球菌（S. mutans）在再生牙釉质上的附着力。使用PAMAM-COOH和PAMAM-NH ₂作为有机模板来诱导牙釉质表面再矿化。再矿化后的矿物沉积物通过扫描电子显微镜（SEM），能量色散光谱（EDS）和X射线衍射（XRD）进行表征。通过原子力显微镜（AFM）分析了再矿化釉质的表面形貌。AFM无尖悬臂使用变形链球菌进行功能化并用作测力探针，以测量细菌与矿化釉质表面之间的粘附力。在变形链球菌悬浮液中孵育24小时后，对矿化釉质表面上的生物膜进行菌落形成单位（CFU）计数。PAMAM-COOH和PAMAM-NH ₂均能在脱矿质的釉质表面上实现有效的再矿化，从而使釉质表面光滑并降低变形链球菌的附着力。PAMAM树状聚合物因其出色的再矿化能力，是用于早期龋齿治疗的有前途的材料。PAMAM树枝状大分子诱导的再矿化使表面光滑并降低变形链球菌的粘附，这可以预防继发性龋齿。