Anaerobic digestion is one of the sustainable processes for biogas production, especially if some of the classic substrate is replaced with lignocellulosic biomass. The presence of lignin makes plant biomass degradation less efficient. Lignin mineralization is an enzyme-dependent process catalyzed by complex ligninolytic enzymes which are produced by white-rot fungi. In our study Pleurotus ostreatus and Trametes versicolor fungi were selected for pre-treatment of Miscanthus which was used as a co-substrate in anerobic digestion. Anaerobic digestion was performed once with ordinary Miscanthus and then with fungi pre-treated Miscanthus. Different process parameters were tested; chicken manure with sawdust and Miscanthus mass ratio (80:20, 60:40, 50:50) and process temperature (35, 40, 42)°C. The volume of biogas produced and the concentration of methane were measured over 21 days. The maximum obtained concentration of methane in the resulting biogas varied between 51% and 58.73% under all different conditions. We have clearly shown that the rate of lignin degradation and consequently the methane productivity are not necessarily improved by fungi pre-treatment. Nevertheless, digestion kinetics is important for designing digesters and predicting the behavior of anaerobic systems. Kinetic parameters of anaerobic digestion of chicken manure with sawdust and ordinary Miscanthus or pre-treated Miscanthus were determined.

厌氧消化是沼气生产的可持续过程之一，特别是如果某些经典底物被木质纤维素生物质代替。木质素的存在使植物生物量的降解效率降低。木质素矿化是由白腐真菌产生的复杂木质素分解酶催化的酶依赖性过程。在我们的研究中，平菇和云芝Trametes选择真菌进行芒草的预处理，将其用作有氧消化中的共底物。用普通的芒草进行一次厌氧消化，然后用真菌预处理的芒草进行厌氧消化。测试了不同的工艺参数；鸡粪与木屑和芒草质量比（80:20，60:40，50:50）和加工温度（35，40，42）°C。在21天内测量了产生的沼气量和甲烷浓度。在所有不同条件下，所得沼气中甲烷的最大最高浓度在51％和58.73％之间变化。我们已经清楚地表明，真菌预处理不一定能提高木质素的降解速度，因此不会提高甲烷的生产率。不过，消化动力学对于设计消化池和预测厌氧系统的行为很重要。测定了锯末和普通芒草或预处理芒草厌氧消化鸡粪的动力学参数。