is necessary for germ cell development in a diverse range of organisms and homologues have been commonly used to identify primordial germ cells (PGCs) during embryogenesis. Here, we isolated full-length cDNA of ortholog in the Pacific oyster and characterized its expression patterns (like) along with analyzing the expression alternation in the gonad of diploids compared with those sterile triploids. qPCR showed that the transcript of like was mainly restricted to the gonad in diploids with ovarian tissues of triploids showing the highest expression. hybridization revealed that -like was found in both female and male gonad where the strongest expression was shown in the germ cells at early stages with no signal in somatic cells. Whole-mount hybridization suggested -like was maternally deposited and the localization of -like mRNA in mesodermal cells might be the putative PGCs in These results suggest that -like was involved in germ line formation, differentiation, and maintenance of germ cells in The obtained findings provide valuable evidences to further facilitate identification of the putative PGCs in the Pacific oyster using -like as a molecular marker.

中文翻译：

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Piwi样基因的表达模式暗示了太平洋牡蛎Crossosrea gigas种系发育中的潜在作用


对于多种生物体的生殖细胞发育是必需的，同源物通常用于在胚胎发生过程中识别原始生殖细胞（PGC）。在这里，我们分离了太平洋牡蛎中直向同源物的全长 cDNA，并表征了其表达模式（类似），同时分析了二倍体与不育三倍体性腺中的表达交替。 qPCR结果显示，like的转录主要局限于二倍体的性腺，三倍体的卵巢组织表达量最高。杂交显示，在雌性和雄性性腺中都发现了-like，其中在早期阶段的生殖细胞中表现出最强的表达，而在体细胞中没有信号。整体杂交表明-like是母体沉积的，并且中胚层细胞中的-like mRNA定位可能是假定的PGCs。这些结果表明-like参与了生殖细胞的生殖系形成、分化和维持。为进一步促进使用-like作为分子标记来鉴定太平洋牡蛎中假定的PGC提供了有价值的证据。