To evaluate functions of vitellogenin (Vg) and heat shock protein 90 (Hsp90) during ovarian and embryonic development of Exopalaemon carinicauda, the cDNA of Vg (Ec-Vg) was cloned, and the abundances of Ec-Vg and heat shock protein 90 (Hsp90) (Ec-Hsp90) were determined during ovarian and embryonic development. During ovarian development, the concentration of Vg protein in hemolymph was markedly greater than in the ovary and hepatopancreas, and was greatest at sexual maturity. The relative abundance of Ec-Vg mRNA transcript was greatest in the hepatopancreas and almost undetectable in hemocytes. By combining mRNA transcript relative abundances with morphological results, a model was developed to explain the mRNA transcript relative abundance of Ec-Hsp90 and Ec-Vg during ovarian and embryonic development. The relative abundance of Ec-Vg mRNA transcript was greatest during the recovery period after reproduction had occurred (Stage V) and mature stage (Stage IV) in the ovary or hepatopancreas, respectively. There were marked associations of the patterns of Ec-Hsp90 and Ec-Vg mRNA transcript abundances both in hepatopancreas and ovary. During embryonic development, the relative abundance of the two mRNA transcripts were greatest at the metazoea and protozoea stages, respectively. These results indicate that Ec-Vg is produced primarily in the hepatopancreas, secreted into the hemolymph and transported into growing oocytes. It, therefore, is believed that Ec-Vg has an important function in the overall ovarian development and late embryonic development of E. carinicauda. In contrast, Ec-Hsp90 is a regulatory factor for Vg transcription and is important during early organogenesis in E. carinicauda.

为评价卵黄蛋白原（Vg）和热休克蛋白90（Hsp90）在鱼鳞虾卵巢和胚胎发育过程中的功能，克隆了Vg（Ec-Vg）的cDNA，并测定了Ec-Vg和热休克蛋白90的丰度（ Hsp90) ( Ec - Hsp90 ) 在卵巢和胚胎发育期间测定。在卵巢发育过程中，血淋巴中 Vg 蛋白的浓度明显高于卵巢和肝胰腺，并且在性成熟时最高。Ec-Vg的相对丰度mRNA 转录在肝胰腺中最大，而在血细胞中几乎检测不到。通过与形态学结果相结合mRNA转录的相对丰度，一个模型来解释的mRNA转录相对丰度的Ec -的Hsp90和EC-Vg的卵巢和胚胎发育过程中。Ec-Vg mRNA 转录物的相对丰度在卵巢或肝胰腺中分别发生繁殖后的恢复期（V 期）和成熟期（IV 期）最大。Ec - Hsp90和Ec-Vg模式之间存在显着关联肝胰腺和卵巢中的 mRNA 转录本丰度。在胚胎发育过程中，两种 mRNA 转录物的相对丰度分别在后生动物和原生动物阶段最大。这些结果表明 Ec-Vg 主要在肝胰腺中产生，分泌到血淋巴中并转运到生长中的卵母细胞中。因此，据信，Ec-Vg 在E. carinicauda的整体卵巢发育和晚期胚胎发育中具有重要作用。相比之下，Ec - Hsp90 是Vg转录的调节因子，并且在E. carinicauda 的早期器官发生过程中很重要。