Herein, a dual-selective recognition and multi-enhanced surface-enhanced Raman scattering (SERS)-fluorescence dual mode detection platform is designed for the detection of circulating tumor cells (CTCs). The gold nanoflowers (AuNFs) substrate was synthesized and the CTCs were captured on the surface area of AuNFs/ITO substrate by aptamers modified. At the same time, the novel nanoprobe was designed, anti-EpCAM (AE) and trigger DNA were modified onto the surface of gold nanostars (AuNSs) through a PEG linker. The novel nanoprobe identified CTCs through the specific recognition reaction between AE and the cell epithelial adhesion molecule of the CTCs. The dual-recognition cellular mechanism of the aptamers and AE improves selectivity. Then, the complementary sequence (CS) hybridize with aptamers to release the captured CTCs into the culture medium. The number of CTCs released was detected by SERS and fluorescence. The limit of SERS detection was 5 cells/mL with a linear relationship from 5 to 200 cells/mL. The limit of fluorescence detection was 10 cells/mL with a linear relationship from 10 to 200 cells/mL. Thus, the developed CTCs detection platform demonstrates promising applications for clinical diagnosis.

中文翻译：

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基于多功能金纳米材料和双选择性识别的特定捕获释放模式循环肿瘤细胞 (CTC) 的 SERS 和荧光检测

在此，设计了一种双选择性识别和多增强表面增强拉曼散射（SERS）-荧光双模式检测平台，用于检测循环肿瘤细胞（CTC）。合成了金纳米花 (AuNFs) 底物，并通过修饰的适体将 CTCs 捕获在 AuNFs/ITO 底物的表面积上。同时，设计了新型纳米探针，通过PEG接头将抗EpCAM（AE）和触发DNA修饰到金纳米星（AuNSs）表面。新型纳米探针通过 AE 与 CTC 的细胞上皮粘附分子之间的特异性识别反应来识别 CTC。适配子和 AE 的双重识别细胞机制提高了选择性。然后，互补序列 (CS) 与适体杂交，将捕获的 CTC 释放到培养基中。通过 SERS 和荧光检测释放的 CTC 数量。SERS 检测限为 5 个细胞/mL，线性关系为 5 至 200 个细胞/mL。荧光检测限为 10 个细胞/mL，线性关系为 10 至 200 个细胞/mL。因此，开发的 CTC 检测平台展示了在临床诊断中的应用前景。