In this work, a novel ecofriendly optical nanosensor for detection of Eplerenone (EPL) in biological samples was reported. Highly luminescent water-soluble nitrogen and sulfur doped carbon quantum dots (N, S-CQDs) have been prepared successfully. The synthesis was based on the reaction of thiosemicarbazide (TS) as source of N and S and citric acid (CA) as source of carbon in one-step aqueous base reflux treatment. The produced N, S-CQDs have a small particle size in the range of 4.7 nm with a high quantum yield (58.5%) and high emission intensity at 446 nm under excitation wavelength of 370 nm. The unique properties of N, S-CQDs make them useful tool as a nano fluorescent probe for sensitive determination of EPL. EPL has been found to decrease the fluorescence of S, N-CDs significantly through static quenching according to the Stern – Volmer plot. The decreased intensity of S, N-CDs fluorescence was proportional to EPL in the 0.2–3.0 μM range. The limit of detection and quantitation were 0.05 and 0.15 μM, respectively. The assay of EPL by this approach was successfully done in drug formulations and in spiked human serum samples.

在这项工作中，报道了一种新型环保光学纳米传感器，用于检测生物样品中的依普利酮（EPL）。成功制备出高发光水溶性氮硫掺杂碳量子点（N，S-CQD）。该合成基于氨基硫脲(TS)作为N和S源以及柠檬酸(CA)作为碳源在一步水碱回流处理中的反应。所制备的N,S-CQD具有4.7 nm范围内的小粒径、高量子产率（58.5%）和在370 nm激发波长下446 nm处的高发射强度。 N, S-CQD 的独特性质使其成为灵敏测定 EPL 的纳米荧光探针的有用工具。根据 Stern – Volmer 图，已发现 EPL 通过静态猝灭显着降低 S、N-CD 的荧光。 S、N-CD 荧光强度的降低与 0.2–3.0 μM 范围内的 EPL 成正比。检测限和定量限分别为0.05 和0.15 μM。通过这种方法成功地在药物制剂和加标人血清样品中进行了 EPL 测定。