In this work, a novel ecofriendly optical nanosensor for detection of Eplerenone (EPL) in biological samples was reported. Highly luminescent water-soluble nitrogen and sulfur doped carbon quantum dots (N, S-CQDs) have been prepared successfully. The synthesis was based on the reaction of thiosemicarbazide (TS) as source of N and S and citric acid (CA) as source of carbon in one-step aqueous base reflux treatment. The produced N, S-CQDs have a small particle size in the range of 4.7 nm with a high quantum yield (58.5%) and high emission intensity at 446 nm under excitation wavelength of 370 nm. The unique properties of N, S-CQDs make them useful tool as a nano fluorescent probe for sensitive determination of EPL. EPL has been found to decrease the fluorescence of S, N-CDs significantly through static quenching according to the Stern – Volmer plot. The decreased intensity of S, N-CDs fluorescence was proportional to EPL in the 0.2–3.0 μM range. The limit of detection and quantitation were 0.05 and 0.15 μM, respectively. The assay of EPL by this approach was successfully done in drug formulations and in spiked human serum samples.

在这项工作中，报道了一种用于检测生物样品中依普利酮 (EPL) 的新型环保光学纳米传感器。已经成功制备了高发光水溶性氮和硫掺杂的碳量子点（N，S-CQDs）。该合成基于氨基硫脲 (TS) 作为 N 和 S 源与柠檬酸 (CA) 作为碳源在一步含水碱回流处理中的反应。产生的 N, S-CQDs 具有 4.7 nm 范围内的小粒径，在 370 nm 激发波长下具有高量子产率 (58.5%) 和 446 nm 的高发射强度。N, S-CQD 的独特性质使其成为一种有用的工具，可用作灵敏测定 EPL 的纳米荧光探针。根据 Stern-Volmer 图，已发现 EPL 通过静态淬灭显着降低 S、N-CD 的荧光。S、N-CDs 荧光强度的降低与 0.2-3.0 μM 范围内的 EPL 成正比。检测限和定量限分别为 0.05 和 0.15 μM。通过这种方法成功地在药物制剂和加标人血清样品中进行了 EPL 分析。