Penetration of a microelectrode into the cell soma is one of the most vulnerable stages of the method intracellular recording. Traumatic neuronal activity after piercing the membrane with a microelectrode can be an indicator of neuronal damage. In this paper we report the results from tests of our pulse electromagnetic micromanipulator. This device is designed to reduce the neuronal damage during the insertion of a microelectrode and to allow the penetration into the cell to be more controlled. Intracellular recordings were employed in experiments carried out on neurons of the isolated central nervous system of the grape snail (Helix pomatia). The results obtained by using our micromanipulator show the absence of a pronounced neuronal reaction in response to the invasion of a microelectrode, the cell rapidly return to a stable state, and registration of neuronal activity is possible for a long period of time (many hours). These findings indicate the relevance of using the proposed device in experiments that record intracellular activity of the cells of the nervous system and other excitable tissues.

中文翻译：

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将微电极插入神经元造成的损伤降至最低的方法

微电极进入细胞体是细胞内记录方法最脆弱的阶段之一。用微电极刺穿膜后的创伤性神经元活动可以是神经元损伤的指标。在本文中，我们报告了脉冲电磁显微操作器的测试结果。该装置旨在减少微电极插入过程中的神经元损伤，并允许对细胞的渗透进行更多控制。在对葡萄蜗牛（Helix pomatia）的分离的中枢神经系统的神经元进行的实验中采用细胞内记录。使用我们的显微操作器获得的结果表明，没有明显的神经元反应来响应微电极的侵入，细胞迅速恢复到稳定状态，并且可以长时间（数小时）记录神经元活动。这些发现表明在记录神经系统细胞和其他可兴奋组织的细胞内活动的实验中使用所提出的装置的相关性。