The liver is one of the body’s tissues that has regenerative abilities. But if the damage is too much, it needs to medical interventions for the regeneration. Liver donor shortage causes researchers to turn to other treatments. Tissue engineering is a new approach to liver regeneration. Hydrogels are polymeric networks of hydrophilic, flexible, and similar to natural tissue. Therefore, they are used to encapsulate cells These constructs are potent substrates to induce differentiation of stem cells to the hepatocytes. According to inadequate availability of the hepatocytes, an alternative cell is required to produce hepatocyte-like cells. Due to the self-renewal and differentiation properties of stem cells, they are suitable cell sources to replace the lostcells. This review has focused on liver regeneration, advantages and disadvantages of hydrogels for liver regeneration, injectable materials, hydrogel fabrication methods, including 3D printing, and stem cells for liver regeneration. Furthermore, this paper shows in vitro, preclinical, and clinical trial studies of hydrogel and stem cells for liver regeneration.

肝脏是人体具有再生能力的组织之一。但如果损伤太大，则需要医疗干预才能再生。肝脏供体短缺导致研究人员转向其他治疗方法。组织工程是一种新的肝脏再生方法。水凝胶是亲水性、柔韧且类似于天然组织的聚合物网络。因此，它们用于包裹细胞。这些构建体是诱导干细胞向肝细胞分化的有效底物。由于肝细胞的可用性不足，需要另一种细胞来产生肝细胞样细胞。由于干细胞的自我更新和分化特性，它们是替代丢失细胞的合适细胞来源。本综述重点关注肝脏再生，用于肝再生的水凝胶、可注射材料、水凝胶制造方法（包括 3D 打印）和用于肝再生的干细胞的优缺点。此外，本文还展示了水凝胶和干细胞用于肝脏再生的体外、临床前和临床试验研究。