Abstract

Effects of 0.1 pM melatonin, 1.0 nM sodium selenite (\({\text{SeO}}_{3}^{{2 - }}\)), and the combined application of these agents on morphogenesis and metabolism of two callus cell lines of Saussurea orgaadayi (Khanm.) Krasnob. were examined. Phenotypic distinctions were revealed for cell cultures obtained from different explants, namely, hypocotyls and cotyledons. The number and dimensions of large-sized cells were higher in the hypocotyl-derived cell culture (HCC) than in the cotyledonary cell culture (CCC). The relation between the growth of two callus cultures and specific traits of their metabolism was noted. The actively growing HCC differed from CCC by a higher content of proline (2.5-fold increase) and ecdysterone-like compound (2.3-fold rise), an increased rate of lipid peroxidation (twofold), and by a lower content of flavonoids. Accordingly, the CCC line exhibited a threefold higher level of flavonoids compared to HCC and a lower content of other compounds. In both cell lines treated with exogenous melatonin, the growth index calculated on a fresh weight basis was increased throughout 20 days after subculture, whereas the presence of \({\text{SeO}}_{3}^{{2 - }}\) elevated the growth index on the 20th–25th days in the CCC line and did not affect the growth index in the HCC line. The combined treatment with melatonin and \({\text{SeO}}_{3}^{{2 - }}\) raised the growth index starting from the 15th day; the stimulatory influence was larger in the HCC than in the CCC line. Melatonin and \({\text{SeO}}_{3}^{{2 - }}\) had a regulatory action on the cellular antioxidant system. Melatonin retarded lipid peroxidation and elevated proline content in both lines. The influence of \({\text{SeO}}_{3}^{{2 - }}\) was less effective than that of melatonin. Under combined action of both factors, changes in lipid peroxidation in CCC were smaller than under the action of melatonin alone, whereas changes in the proline content were enhanced. The effect of the combined treatment on lipid peroxidation and proline content in HCC did not differ from the effects exerted by melatonin alone. Melatonin and \({\text{SeO}}_{3}^{{2 - }}\) were hypothesized to differentially affect the cell culture respiration as a source of reactive oxygen species.

中文翻译：

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褪黑素和硒调节来自不同植株的雪莲的体外生长和氧化状态。

摘要

0.1 pM褪黑素，1.0 nM亚硒酸钠（\（{\ text {SeO}} _ {3} ^ {{2-}} \））的作用，以及这些试剂联合应用对两个愈伤组织细胞的形态发生和代谢的影响行雪莲orgaadayi（汗）克拉斯诺布。被检查。从不同外植体，即胚轴和子叶获得的细胞培养物表现出表型差异。下胚轴来源的细胞培养物（HCC）中的大细胞的数量和大小要比子叶细胞培养物（CCC）中的大。注意到两种愈伤组织培养物的生长与其代谢的特定性状之间的关系。活跃生长的HCC与CCC的区别在于脯氨酸含量较高（增加2.5倍）和类蜕皮甾酮类化合物（含量增加2.3倍），脂质过氧化率增加（两倍）和类黄酮含量较低。因此，与HCC相比，CCC品系的类黄酮含量高出三倍，而其他化合物的含量则更低。在用外源褪黑激素处理的两种细胞系中，\（{\ text {SeO}} _ {3} ^ {{2-}} \}提高了CCC行中第20-25天的生长指数，而没有影响HCC行中的生长指数。从第15天开始，褪黑激素和\（{\ text {SeO}} _ {3} ^ {{2-}} \}的联合处理提高了生长指数；HCC的刺激作用大于CCC线。褪黑激素和\（{\ text {SeO}} _ {3} ^ {{2-}} \）对细胞抗氧化剂系统具有调节作用。褪黑素可延缓两种系的脂质过氧化和脯氨酸含量的升高。的影响\（{\ {文本的SeO}} _ {3} ^ {{2 - }} \）不如褪黑素有效。在这两种因素的共同作用下，CCC中脂质过氧化的变化要比单独使用褪黑激素时小，而脯氨酸含量的变化则增加。联合治疗对HCC脂质过氧化和脯氨酸含量的影响与单独褪黑激素所产生的影响没有区别。假设褪黑激素和\（{\ text {SeO}} _ {3} ^ {{2-}} \）可以差异地影响细胞培养物呼吸作为活性氧的来源。