Abstract

Cardiocrinum cathayanum (Endl.) Lindl. (Liliaceae) is a promising species for ornamental and pharmaceutical usage. However, genomic responses of C. cathayanum to temperature have not been investigated. In the present study, C. cathayanum leaves were cultivated at 10, 20, and 30°C, and leaves were collected for transcriptome sequencing. Overall, 36.9 to 44.1 M clean reads were obtained, which finally assembled 48 120 unigenes. Among these unigenes, 49.39% could be annotated to at least one public database. Compared with 20°C treatment, 381 and 303 unigenes were significantly upregulated, 580 and 1399 were significantly downregulated in 10 and 30°C treatments, respectively. Real-time qPCR analyses on selected 12 unigenes showed similar tendencies to those calculated on transcriptome sequencing. Compared with 20°C treatment, exposure to 10°C significantly affected the pentose and glucuronate interconversions, starch and sucrose metabolism pathways. Further exploration suggested that D-galacturonate and trehalose were accumulated at 10°C, which would enhance the tolerance to cold stress. Besides, 10°C treatment inhibited the biosynthesis of unsaturated fatty acids and α-linolenic acid metabolism. These changes might negatively affect C. cathayanum growth. At 30°C, 40 of 42 differentially expressed genes (DEGs) in the starch and sucrose metabolism, all the 27 DEGs in the glycolysis/gluconeogenesis and all the 17 DEGs in the fructose and mannose metabolism were significantly downregulated, in comparison to 20°C treatment. These changes demonstrated that high temperature greatly inhibited carbohydrate metabolism in C. cathayanum leaves. Overall, the present study contributed new insights to understand the genomic responses of C. cathayanum leaves to various temperatures.

中文翻译：

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温度对心柳叶片转录组谱的影响

摘要

Cardiocrinum cathayanum（Endl。）Lindl。（紫ilia科）是用于观赏和药物用途的有前途的物种。但是，尚未研究C. cathayanum对温度的基因组响应。在本研究中，C。cathayanum在10、20和30°C下培养叶片，并收集叶片进行转录组测序。总体而言，获得了36.9至44.1 M的干净读取，最终组装了48 120个单基因。在这些单基因中，至少有一个公共数据库可以注释49.39％。与20°C处理相比，在10和30°C处理中，分别有381和303个单基因显着上调，而在580和1399中显着下调。对选定的12个单基因进行的实时qPCR分析显示出与转录组测序所计算出的趋势相似的趋势。与20°C处理相比，暴露于10°C显着影响戊糖和葡萄糖醛酸酯的相互转化，淀粉和蔗糖的代谢途径。进一步的研究表明D-半乳糖醛酸和海藻糖在10°C时积累，这将增强对冷胁迫的耐受性。此外，10°C处理会抑制不饱和脂肪酸的生物合成和α-亚麻酸的代谢。这些变化可能会产生负面影响C. cathayanum生长。在30°C下，淀粉和蔗糖代谢中的42个差异表达基因（DEG）中有40个，与20°相比，糖酵解/糖原异生中的所有27个DEG以及果糖和甘露糖代谢中的所有17个DEG被显着下调。 C治疗。这些变化表明高温极大地抑制了矢车菊叶片中的碳水化合物代谢。总的来说，本研究为了解C. cathayanum叶片对各种温度的基因组反应做出了新的贡献。