In this study, a micropropagation protocol using nodal explants from in vitro grown plants of Chamerion angustifolium (L.) Holub was developed and analysis of oenothein B and selected phenolic acids in shoot cultures was performed for the first time. For shoot induction and multiplication Murashige and Skoog’s (MS) basal medium supplemented with 2-isopentenyladenine (2iP), zeatin (Z) and 6-benzyloaminopurine (BAP) was used. 2iP was the most responsive in terms of promoting shoots per explant with the maximum (6.57 ± 1.14) recorded at a concentration of 2.0 mg L⁻¹ after 6 weeks of culture. After two subcultures the multiplication rate was increased up to 19 shoots per explant on medium with 2iP (1.0 mg L⁻¹). To prevent tissue browning, ascorbic acid and casein hydrolysate were added to the induction medium, resulting in a reduction of browning by 30%. The rooted plantlets were successfully transferred to soil and acclimatized with 97% frequency. Quantitative and qualitative assessments of oenothein B and phenolic acid contents in in vitro regenerated shoots as well as in ex vitro plants were performed using high-performance liquid chromatography with a diode-array detector (HPLC-DAD) and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC MS/MS) methods. Oenothein B (1.62‒4.55 g 100 g⁻¹ DW), ellagic acid, gallic and caffeic acids were identified in in vitro regenerated plants. The results of this study confirm that the oenothein B-producing plantlets can be obtained using the micropropagation method with axillary shoots being a valuable source of oenothein B and phenolic acids.

在这项研究中，开发了使用来自Chamerion angustifolium（L.）Holub的离体生长植物的节点外植体的微繁殖方案，并且首次进行了茎培苗中皂苷B和所选酚酸的分析。对于芽的诱导和繁殖，使用补充有2-异戊烯腺嘌呤（2iP），玉米蛋白（Z）和6-苄基氨基嘌呤（BAP）的Murashige和Skoog's（MS）基础培养基。在培养6周后，就每个外植体的促生芽而言，2iP的响应最强，在2.0 mg L ^-1的浓度下记录的最大值为（6.57±1.14）。经过两次传代培养，在2iP（1.0 mg L ^-1）。为防止组织褐变，将抗坏血酸和酪蛋白水解物添加到诱导培养基中，从而使褐变减少30％。生根的小苗成功地转移到土壤中，并以97％的频率适应环境。高效液相色谱-二极管阵列检测器（HPLC-DAD）和超高效液相色谱-串联质谱法对离体再生芽和离体植物中的皂苷B和酚酸含量进行了定量和定性评估质谱（UPLC MS / MS）方法。皂甙B（1.62‒4.55 g 100 g ^-1在体外再生植物中鉴定出鞣花酸，没食子酸，没食子酸和咖啡酸。这项研究的结果证实，使用微繁繁殖法可以得到产卵泡蛋白B的小植株，而腋生芽是卵泡蛋白B和酚酸的重要来源。