Cotton (Gossypium hirsutum L.), a mercantile crop plant, is grown worldwide for fiber and seed oil. As with other economically important crops, cotton is bogged down with many biotic and abiotic stress factors. Towards this, genetic engineering offers numerous protocols to engineer plants for better resilience. However, recalcitrance of cotton to plant tissue culture has been the major constraint for successful in vitro regeneration. Hence, alternate methods that evade tissue culture regeneration have been envisaged. Non tissue culture-based in planta transformation strategies are in vogue due to amenability and ease in the generation of transgenic plants. In the present study, we demonstrate the utility of an in planta transformation protocol and establishment of a stringent selection agent-based screening for the identification of transgenics. The genotype independent nature of the protocol was validated in cotton cv. Pusa 8–6 using GFP. Preliminary transformation efficiency of 28% was achieved with a screening efficiency of 20% in the presence of hygromycin. The proof of T-DNA integration by various molecular and expression analysis in T1 and T2 generations proved that this technique can be employed to generate transgenic cotton.

棉花 ( Gossypium hirsutum L.) 是一种商业作物植物，在世界范围内种植以获取纤维和种子油。与其他具有重要经济意义的作物一样，棉花也受到许多生物和非生物胁迫因素的影响。为此，基因工程提供了许多协议来设计植物以获得更好的恢复力。然而，棉花对植物组织培养的抵抗一直是体外再生成功的主要制约因素。因此，已经设想了逃避组织培养再生的替代方法。由于转基因植物产生的适应性和简便性，基于非组织培养的植物转化策略很流行。在本研究中，我们展示了一种在植物中的效用转化方案和建立严格的基于选择剂的筛选以鉴定转基因。该协议的基因型独立性质在棉花 cv 中得到验证。Pusa 8-6 使用GFP。在潮霉素存在下，初步转化效率为 28%，筛选效率为 20%。通过T1和T2代的各种分子和表达分析证明T-DNA整合证明该技术可用于产生转基因棉花。