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Identification and characterization of white onion ( Allium cepa L.) genotypes for high total soluble solid content through molecular markers
Genetic Resources and Crop Evolution ( IF 1.6 ) Pub Date : 2020-10-19 , DOI: 10.1007/s10722-020-01037-9
Puthem Robindro Singh , Vijay Mahajan , Annu Verma , R. Sagar , Kuldip Jayaswall , Neeraj Shukla , Pranjali Gedam , Major Singh

Onion (Allium cepa L.) is one of the most important bulbous vegetable used in global kitchen. Although it has various important traits but among them the high total soluble solid content (HTSS) of white onion is highly preferred by the industries for processing purpose. Looking into the broader use of the white onion; we utilized a total of 23 polymorphic simple sequence repeats (SSRs) and intron length polymorphic markers (ILPs) to characterize thirty five white onion genotypes for HTSS. Further, we also correlated these DNA fingerprint data with the TSS for identification of HTSS and LTSS lines. The total average numbers of alleles for SSR and ILP locus, heterozygosity (He) and polymorphism information content were found to be 2.4, 0.35 and 0.29 respectively. The UPGMA dendrogram revealed two distinct clusters of genotypes. Based on the TSS and DNA genotype data, our study revealed that Bhima-shweta (LTSS-12.09%, sub-cluster IAa) & WHT-12L-HT-15-Reject-M-7(HTSS- 18.02%, cluster II) are more diverse than the others. Other white onion lines including WHTB-7G-GT-15-SC-M-7 small bulb (HTSS 18.80%), WHT-2B-GT-18-SC-M-7 (HTSS 18.51%), WHTS-4D-GT-18-MC-M-7 (HTSS 18.49%), WHTB-3C-GT-18-MC-M-7 (HTSS 18.27%) and WHTS-11K-Pickle-SC-M-7 (HTSS 17.68%) were identified as superior HTSS lines. These identified diverse HTSS and LTSS lines could be useful for the mapping of HTSS coding genes for the acceleration of molecular breeding of onion through the marker assisted selection (MAS) which could be used by the industries for the larger scale processing of the white onion products required by the global community.



中文翻译:

通过分子标记鉴定和鉴定高总可溶性固形物含量的白洋葱(Allium cepa L.)基因型

洋葱(洋葱)L.)是全球厨房中使用的最重要的球根蔬菜之一。尽管它具有各种重要的特性,但其中高白洋葱的总可溶性固形物(HTSS)被工业高度推荐用于加工目的。研究白洋葱的广泛用途;我们利用总共23个多态性简单序列重复(SSR)和内含子长度多态性标记(ILP)来表征HTSS的35个白洋葱基因型。此外,我们还将这些DNA指纹数据与TSS相关联,以鉴定HTSS和LTSS品系。SSR和ILP基因座的等位基因总数平均,杂合性(He)和多态性信息含量分别为2.4、0.35和0.29。UPGMA树状图显示了两个不同的基因型簇。根据TSS和DNA基因型数据,我们的研究表明,Bhima-shweta(LTSS-12.09%,亚类IAa)和WHT-12L-HT-15-Reject-M-7(HTSS-18.02%,II类)比其他类型更具多样性。其他白洋葱线包括WHTB-7G-GT-15-SC-M-7小鳞茎(HTSS 18.80%),WHT-2B-GT-18-SC-M-7(HTSS 18.51%),WHTS-4D-GT -18-MC-M-7(HTSS 18.49%),WHTB-3C-GT-18-MC-M-7(HTSS 18.27%)和WHTS-11K-Pickle-SC-M-7(HTSS 17.68%)为被确定为高级HTSS品系。这些鉴定出的不同的HTSS和LTSS品系可用于HTSS编码基因的定位,以通过标记辅助选择(MAS)加速洋葱的分子育种,该技术可被工业用于大规模加工白洋葱产品。全球社区的要求。WHT-12L-HT-15-Reject-M-7(HTSS-18.02%,类集II)比其他类更多样化。其他白洋葱线包括WHTB-7G-GT-15-SC-M-7小鳞茎(HTSS 18.80%),WHT-2B-GT-18-SC-M-7(HTSS 18.51%),WHTS-4D-GT -18-MC-M-7(HTSS 18.49%),WHTB-3C-GT-18-MC-M-7(HTSS 18.27%)和WHTS-11K-Pickle-SC-M-7(HTSS 17.68%)为被确定为高级HTSS品系。这些鉴定出的不同的HTSS和LTSS品系可用于HTSS编码基因的定位,以通过标记辅助选择(MAS)加速洋葱的分子育种,该技术可被工业用于大规模加工白洋葱产品。全球社区的要求。WHT-12L-HT-15-Reject-M-7(HTSS-18.02%,类集II)比其他类更多样化。其他白洋葱线包括WHTB-7G-GT-15-SC-M-7小鳞茎(HTSS 18.80%),WHT-2B-GT-18-SC-M-7(HTSS 18.51%),WHTS-4D-GT -18-MC-M-7(HTSS 18.49%),WHTB-3C-GT-18-MC-M-7(HTSS 18.27%)和WHTS-11K-Pickle-SC-M-7(HTSS 17.68%)为被确定为高级HTSS品系。这些鉴定出的不同的HTSS和LTSS品系可用于HTSS编码基因的定位,以通过标记辅助选择(MAS)加速洋葱的分子育种,该技术可被工业用于大规模加工白洋葱产品。全球社区的要求。WHTS-4D-GT-18-MC-M-7(HTSS 18.49%),WHTB-3C-GT-18-MC-M-7(HTSS 18.27%)和WHTS-11K-Pickle-SC-M-7( HTSS 17.68%)被鉴定为优良的HTSS品系。这些鉴定出的不同的HTSS和LTSS品系可用于HTSS编码基因的定位,以通过标记辅助选择(MAS)加速洋葱的分子育种,该技术可被工业用于大规模加工白洋葱产品。全球社区的要求。WHTS-4D-GT-18-MC-M-7(HTSS 18.49%),WHTB-3C-GT-18-MC-M-7(HTSS 18.27%)和WHTS-11K-Pickle-SC-M-7( HTSS 17.68%)被鉴定为优良的HTSS品系。这些鉴定出的不同的HTSS和LTSS品系可用于HTSS编码基因的定位,以通过标记辅助选择(MAS)加速洋葱的分子育种,该技术可被工业用于大规模加工白洋葱产品。全球社区的要求。

更新日期:2020-10-19
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