Croatian isolates of Monilinia fructicola, M. laxa and M. fructigena have been collected from peach, nectarine, sweet cherry, plum and apricot fruits and assessed for their sensitivity to thiophanate-methyl. Out of 66 isolates collected, 34 were identified as M. fructicola (52%), 22 as M. fructigena (33%) and 10 as M. laxa (15%). Mycelial radial growth on water agar amended with thiophanate-methyl was measured to determine half maximal effective concentration values (EC₅₀). All isolates of M. laxa and M. fructigena showed as sensitive, with EC₅₀ values lower than 1 µg ml^− 1 in 31 from 32 isolates. In M. fructicola, 19 isolates (56%) were sensitive, nine (26%) were resistant (EC₅₀ 2–30 µg ml^− 1), and six (18%) were highly resistant (EC₅₀ > 30 µg ml^− 1). Positive allele-specific PCR with primer pair HRR/HRF, detecting resistant point mutations in β-tubulin gene, was recorded in 15 out of 16 highly resistant, resistant and less sensitive M. fructicola isolates. Among all species, PCR assay coincided with agar assay in 95% of cases. Highly resistant M. fructicola isolates AL 24/19 and VR 8/18 caused typical brown rot on nectarine fruits inoculated with conidia suspended in 5 or 10 µg ml^− 1 of thiophanate-methyl. Four sensitive isolates of all three species did not develop on inoculated fruits. Besides implications for brown rot management, the finding of M. fructicola strains resistant to thiophanate-methyl may indicate that this species could be adapting to fungicides more rapidly than M. laxa or M. fructigena. As resistant M. fructicola isolates were also found in orchards where benzimidazoles have never been used, there is a possibility they originate from already benzimidazole-resistant strains which entered and established in Croatia.

克罗地亚株的褐腐病菌，中号。疏花和中号。从桃，油桃，甜樱桃，李子和杏果实中收集到了果糖，并对其对甲基硫氰酸酯的敏感性进行了评估。出的收集66株，34被鉴定为中号。腐病菌（52％），22为M. fructigena（33％）和10作为M.疏花（15％）。测量了用甲基硫氰酸酯修饰的水琼脂上的菌丝体径向生长，以确定最大有效浓度值的一半（EC ₅₀）。M的所有分离株。laxa和M。果糖表现出敏感性，32种分离物中的31种EC ₅₀值低于1 µg ml ^-1。在M.腐病菌，19株（56％）是敏感，九（26％）为抗性的（EC ₅₀ 2-30微克毫升^{- 1}），和六（18％）为高抗（EC ₅₀  > 30微克毫升^{- 1}）。在16个高抗性，抗性和敏感性较低的M中，有15个记录了带有引物对HRR / HRF的正等位基因特异性PCR，可检测β-微管蛋白基因中的抗性点突变。果蝇分离物。在所有物种中，有95％的病例PCR分析与琼脂分析相吻合。高抗中号。果糖分离物AL 24/19和VR 8/18导致油桃果实上典型的棕褐色腐烂，而油桃果实中接种了以5或10 µg ml ^{− 1}硫代甲基丙烯酸甲酯悬浮的分生孢子。所有三个物种的四个敏感分离株未在接种的果实上发育。除了为褐腐病管理的影响，人的发现M.腐病菌菌株对甲基硫菌灵抗性可以指示该物种可以更快速地适应于杀真菌剂比M.疏花或M. fructigena。由于在从未使用过苯并咪唑的果园中也发现了抗性毛果支原体分离株，因此它们有可能起源于已经进入克罗地亚并在克罗地亚建立的对苯并咪唑具有抗性的菌株。