Caseous lymphadenitis (CL) is a chronic infectious disease that affects sheep and goats. Many serological tests have been developed to detect the disease; one of the most widely used is the enzyme-linked immunosorbent assay (ELISA), due to its advantages, which include acceptable cost-effectiveness, applicability, sensitivity and specificity. ELISA formulations using recombinant proteins can exhibit significant sensitivity and specificity when using a single purified antigen. DTxR, Trx, TrxR, LexA, SodC, SpaC, NanH, and PknG recombinant proteins can be considered target proteins for ELISA development due to its extracellular or on the cell surface location, which allows a better recognition by the immune system. Therefore, the objectives of this study were to evaluate the antigenic reactivity of Corynebacterium pseudotuberculosis recombinant proteins in goat and sheep serum. Of eight proteins evaluated, rSodC was selected for validation assays with small ruminant serum samples from the semiarid region of the state of Bahia, Brazil. Validation assays with goat serum samples showed that ELISA-rSodC presented sensitivity and specificity of 96% and 94%, respectively. Validation assays with sheep serum showed that ELISA-rSodC exhibited sensitivity and specificity of 95% and 98%, respectively. Analysis of 756 field serum samples showed that rSodC identified 95 positive samples (23%) in goats and 75 positive samples (21%) in sheep. The ELISA with recombinant SodC protein developed in this study discriminated positive and negative serum samples with high levels of sensitivity and specificity. This formulation is promising for epidemiological surveys and CL control programs.

Trial registration AEC No 4958051018. 12/18/2018, retrospectively registered

干酪性淋巴结炎（CL）是一种慢性感染性疾病，会影响绵羊和山羊。已经开发出许多血清学检测方法来检测这种疾病。酶联免疫吸附测定法（ELISA）由于其优点，包括可接受的成本效益，适用性，敏感性和特异性，是使用最广泛的方法之一。当使用单一纯化的抗原时，使用重组蛋白的ELISA制剂可表现出显着的敏感性和特异性。DTxR，Trx，TrxR，LexA，SodC，SpaC，NanH和PknG重组蛋白由于其在细胞外或在细胞表面的位置而可以被认为是ELISA发展的目标蛋白，从而可以更好地被免疫系统识别。因此，本研究的目的是评估甲胎蛋白的抗原反应性。假结核棒杆菌山羊和绵羊血清中的重组蛋白。在评估的八种蛋白质中，选择了rSodC进行来自巴西巴伊亚州半干旱地区的小反刍动物血清样品的验证分析。山羊血清样品的验证分析表明，ELISA-rSodC的敏感性和特异性分别为96％和94％。用绵羊血清进行的验证试验表明，ELISA-rSodC的敏感性和特异性分别为95％和98％。对756个现场血清样品的分析表明，rSodC在山羊中鉴定出95个阳性样品（23％），在绵羊中鉴定出75个阳性样品（21％）。在这项研究中开发的带有重组SodC蛋白的ELISA能够以高水平的灵敏度和特异性区分阳性和阴性血清样品。该配方有望用于流行病学调查和CL控制计划。

试用注册AEC编号4958051018.2018 / 12/18，追溯注册