Perls' Prussian blue (PPB) stain recognizes Fe³⁺ associated with hemosiderin. The employment of this stain in clinical medicine and research has been extensive and novel applications continue to evolve. Ferruginous bodies are intracellular structures in lung tissue, bronchoalveolar lavage (BAL), and sputum that stain with PPB. Inhaled, insoluble, biopersistent particles and fibers are phagocytosed by lung macrophages and thought to be coated, either partially or completely, with an iron-containing protein at the interface forming a ferruginous body. These structures can be categorized as ferruginous bodies having either an inorganic or a carbonaceous core (e.g., asbestos and byssinotic bodies, respectively). In lung tissue, BAL, and sputum, the only cells that stain with PPB are macrophages. These are described as iron- and hemosiderin-laden macrophages and called either siderophages or sideromacrophages. Siderophages can be observed in the lung tissue, BAL, and sputum after various exposures and can also be associated with many different pulmonary and extrapulmonary diseases.

中文翻译：

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肺组织、支气管肺泡灌洗液和痰的 Perls 普鲁士蓝染色

Perls 的普鲁士蓝 (PPB) 染色剂可识别 Fe ³⁺与含铁血黄素有关。这种染色剂在临床医学和研究中的应用非常广泛，新的应用也在不断发展。铁质小体是肺组织、支气管肺泡灌洗液 (BAL) 和用 PPB 染色的痰液中的细胞内结构。吸入的不溶性生物持久性颗粒和纤维被肺巨噬细胞吞噬，并被认为在界面处部分或完全被含铁蛋白质覆盖，形成铁质体。这些结构可归类为具有无机或碳质核心的铁质体（例如，分别为石棉体和无氧体）。在肺组织、BAL 和痰中，唯一被 PPB 染色的细胞是巨噬细胞。这些被描述为富含铁和含铁血黄素的巨噬细胞，称为铁噬菌体或铁巨噬细胞。在各种暴露后，可以在肺组织、BAL 和痰液中观察到嗜铁噬菌体，并且还可能与许多不同的肺部和肺外疾病有关。