Silica-supported dual-dye nanoprobes for ratiometric hypoxia sensing,Materials Chemistry Frontiers

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Silica-supported dual-dye nanoprobes for ratiometric hypoxia sensing
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2020-10-16 , DOI: 10.1039/d0qm00653j
Chang Wang _{1,

2,

3,

4} , Yu-Zhe Chen _{1,

2,

3,

4} , Qing-Zheng Yang _{5,

6,

7,

8,

9} , Chen-Ho Tung _{1,

2,

3,

4} , Da-Yong Wu _{1,

2,

3,

4} , Li-Zhu Wu _{1,

2,

3,

4}

Affiliation

Oxygen (O₂) levels provide an important parameter in the early-stage diagnosis of cancers and other diseases. Ratiometric optical O₂ sensing has attracted much interest due to its better calibration of oxygen levels at two wavelengths. Oxygen sensing nanoprobes are generally designed by the incorporation of a reference dye and phosphorescent indicator into various matrixes. Silica nanomaterials with intriguing characteristic properties are highly preferable in hypoxia sensing. However, physical entrapment of dyes in silica suffers from leaching and moderate sensitivity. Reported herein is a covalently encapsulated dual-dye silica nanoprobe for ratiometric hypoxia detection and imaging. The nanoprobe prepared in a one-pot approach has a core–shell nanostructure where silanized indicators and reference dyes are covalently grafted within the silica core, while poly(ethylene glycol) chains form an outer shell. To our delight, through the covelent bonding of the dyes for efficient energy transfer between the reference dye and the indicator, high phosphorescence brightness, long lifetime and excellent stability of the nanoprobe were achieved. More importantly, the designed nanoprobe can respond to different oxygen levels in both solution and living cells with impressive sensitivity and stability.

中文翻译：

二氧化硅支持的双染料纳米探针，用于比率式缺氧传感

氧气（O ₂）水平在癌症和其他疾病的早期诊断中提供了重要参数。比例光学O ₂传感技术由于能够更好地校准两个波长的氧气水平而引起了人们的极大兴趣。氧感测纳米探针通常通过将参考染料和磷光指示剂掺入各种基质中来设计。具有耐人寻味的特性的二氧化硅纳米材料在缺氧感测中是高度优选的。但是，染料在二氧化硅中的物理俘获存在浸出和中等敏感性的问题。本文报道了一种用于比例缺氧检测和成像的共价封装的双染料二氧化硅纳米探针。通过一锅法制备的纳米探针具有核-壳纳米结构，其中硅烷化指示剂和参考染料共价接枝在二氧化硅核中，而聚乙二醇链形成外壳。令我们高兴的是，通过染料的凹角键合以在参考染料和指示剂之间进行有效的能量转移，实现了纳米探针的高磷光亮度，长寿命和出色的稳定性。更重要的是，设计的纳米探针可以以出色的灵敏度和稳定性响应溶液和活细胞中不同的氧气含量。

更新日期：2020-12-10

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