Carrier culture is the combination of suspension culture and adherent culture. Carriers could be used to improve the culture efficiency of large-scale culture of adherent benthic diatom. In order to identify a suitable carrier for mass suspension culture of the benthic diatoms Nitzschia sp. powders of seven macroalgae were used as carriers for diatom attachment including Ulva pertusa, degummed Laminaria japonica, Sargassum muticum, Chaetomorpha valida, Zostera marina, Sargassum fusiforme and Sargassum thunbergii. Diatoms could grow on the surface of the suspended carriers and could effectively utilize the nutrients released by the decomposition of these carriers. Among the seven carriers, Ulva pertusa powder was the most effective for Nitzschia culture when comprehensively considering the nutrition of the harvested diatoms and nutrient utilization of the algae carrier by diatoms. The seaweed powder was dried at 80 °C before used as carrier, this process should meet the sterilization requirements for microalgal culture and is easy to implement in the large-scale cultivation of diatoms. Diatoms cultured with carriers were used to feed juveniles Stichopus japonicus with a wet weight of 5.0 ± 2.0 g, as well as diatoms cultured using conventional methods without carriers, and no significant differences were observed between the two kinds of feed. Compared to sea cucumbers fed with commercial feed, the growth rate of S. japonicus fed with carrier-cultured diatoms was approximately 1.5 times greater, and the activities of non-specific immune enzymes were improved in the coelomic fluid including acidic phosphatase, alkaline phosphatase and superoxide dismutase. Feeding sea cucumber with fresh diatoms could effectively reduce the total nitrogen and total phosphorus in the culture water, improve the water quality, and promote the breeding of sea cucumbers, regardless of whether the carrier culture was adopted.

中文翻译：

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底栖硅藻Nitzschia sp。的培养。藻类载体的合成及其在少年刺鱼饲料中的应用

载体培养是悬浮培养和贴壁培养的结合。载体可用于提高附着底栖硅藻大规模培养的培养效率。为了确定合适的载体用于底栖硅藻的大量悬浮培养，Nitzschia sp。七个大型藻类的粉末被用作硅藻附着的载体，包括百日草，脱胶的海带，日本羊栖小球藻，Chaetomorpha valida，滨海带状疱疹，Sargassum fusiforme和Sargassum thunbergii。硅藻可以在悬浮的载体表面上生长，并可以有效地利用这些载体分解释放出的养分。在这七家航空公司中，当综合考虑收获的硅藻的营养和硅藻对藻类载体的营养利用时，百日咳粉对尼采培养最有效。该海藻粉在用作载体之前先在80°C下干燥，该工艺应满足微藻培养的灭菌要求，并且易于在硅藻的大规模培养中实施。用带载体培养的硅藻喂养湿重为5.0±2.0 g的日本刺槐幼体，以及使用不带载体的常规方法培养的硅藻，两种饲料之间没有观察到显着差异。与以商业饲料喂养的海参相比，以载体培养的硅藻喂养的日本刺参的生长速度大约高出1.5倍，结肠液中酸性磷酸酶，碱性磷酸酶和超氧化物歧化酶的非特异性免疫酶活性得到改善。用新鲜的硅藻喂养海参可以有效地减少养殖水中的总氮和总磷，改善水质，并促进海参的繁殖，无论是否采用载体培养。