Quantum dots (QDs) are powerful materials in various bioapplications based on their excellent optical and electronic properties. For the application of various fields of QDs, surface modification of QDs is necessary. However, surface modification in QDs may result in a reduction in quantum yield (QY). This reduction of QY causes many weaknesses in the biological application of QDs. In this study, CdSeZnS/ZnS alloy QDs were used to prepare antibody-conjugated QDs for a sandwich immunoassay. The alloy QDs displayed a QY of 84.5% that was maintained at 83.0% (98.2% of QY was maintained) after surface modification with the anti-rabbit IgG as a model study. Surface-modified QDs successfully detected their corresponding target through antibody-antigen binding. The limit of detection was  ng mL^-1 for rabbit IgG.

中文翻译：

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用于免疫分析的稳定CdSeZnS / ZnS合金量子点的表面修饰

量子点（QD）具有出色的光学和电子特性，是各种生物应用中的强大材料。对于量子点的各个领域的应用，量子点的表面改性是必要的。但是，QD中的表面改性可能会导致量子产率（QY）降低。QY的减少导致QD在生物学上的应用中存在许多弱点。在这项研究中，使用CdSeZnS / ZnS合金QD制备用于三明治免疫测定的抗体偶联QD。用抗兔IgG作为模型研究进行表面改性后，合金QD的QY保持在83.0％（保持QY的98.2％），保持在83.0％。经表面修饰的QD通过抗体-抗原结合成功检测到了它们相应的靶标。检出限为 ng mL ^-1（兔IgG）。