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Pre-meiotic, 21-nucleotide Reproductive PhasiRNAs Emerged in Seed Plants and Diversified in Flowering Plants
bioRxiv - Plant Biology Pub Date : 2020-10-17 , DOI: 10.1101/2020.10.16.341925
Suresh Pokhrel , Kun Huang , Sébastien Bélanger , Jeffrey L. Caplan , Elena M. Kramer , Blake C. Meyers

Plant small RNAs (sRNAs) are important regulatory elements that fine-tune gene expression and maintain genome integrity by silencing transposons. They have critical roles in most pathways involved in plant growth and reproductive development. Reproductive organs of monocots produce abundant phased, small interfering RNAs (phasiRNAs). The 21-nt reproductive phasiRNAs triggered by miR2118 are highly enriched in pre-meiotic anthers, and have not been described in eudicots. The 24-nt reproductive phasiRNAs are triggered by miR2275, and are highly enriched during meiosis in many angiosperms. Here, we describe additional variants of 21-nt reproductive phasiRNAs, including those triggered by miR11308 in wild strawberry, a eudicot, and we validate the presence of this pathway in rose. We report the widespread presence of the 21-nt reproductive phasiRNA pathway in eudicots, with novel biogenesis triggers in the basal eudicot columbine and the rosid flax. In eudicots, these 21-nt phasiRNAs are enriched in pre-meiotic stages, a spatiotemporal distribution consistent with that of monocots and suggesting a role in anther development. Although this pathway is apparently absent in well-studied eudicot families including the Brassicaceae, Solanaceae and Fabaceae, our work in eudicots supports a singular finding in spruce, indicating that the pathway of 21-nt reproductive phasiRNAs emerged in seed plants and was lost in some lineages.

中文翻译:

减数分裂前的21个核苷酸的生殖PhasiRNA在种子植物中出现并在开花植物中多样化

植物小RNA(sRNA)是重要的调控元件,可通过沉默转座子来微调基因表达并维持基因组完整性。它们在涉及植物生长和生殖发育的大多数途径中都起着关键作用。单子叶植物的生殖器官产生大量分阶段的小干扰RNA(phasiRNA)。由miR2118触发的21 nt生殖phasiRNA在减数分裂前的花药中高度富集,在双子叶植物中尚未见报道。24-nt生殖phasiRNA由miR2275触发,在许多被子植物的减数分裂过程中高度富集。在这里,我们描述了21-nt生殖phasiRNA的其他变体,包括由野生草莓eudicot中的miR11308触发的变体,并且我们验证了玫瑰中该途径的存在。我们报告了在双子叶植物中21-nt生殖phasiRNA途径的广泛存在,在基底双子叶植物哥伦拜恩和玫瑰色亚麻中有新的生物发生触发因素。在双子叶植物中,这些21-nt phasiRNA在减数分裂前阶段富集,其时空分布与单子叶植物一致,并提示其在花药发育中的作用。尽管在经过深入研究的杜鹃科(包括十字花科,茄科和豆科)中显然没有这种途径,但我们在双子叶植物中的工作支持了在云杉中的发现,这表明21nt生殖phasiRNA的途径出现在种子植物中,并在某些植物中消失了。血统。与单子叶植物一致的时空分布,提示其在花药发育中的作用。尽管在经过深入研究的杜鹃科(包括十字花科,茄科和豆科)中显然没有这种途径,但我们在双子叶植物中的工作支持了在云杉中的发现,这表明21nt生殖phasiRNA的途径出现在种子植物中,并在某些植物中消失了。血统。与单子叶植物一致的时空分布,提示其在花药发育中的作用。尽管在经过深入研究的杜鹃科(包括十字花科,茄科和豆科)中显然没有这种途径,但我们在双子叶植物中的工作支持了在云杉中的发现,这表明21nt生殖phasiRNA的途径出现在种子植物中,并在某些植物中消失了。血统。
更新日期:2020-10-17
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