C₄ photosynthesis is characterized by the compartmentalization of the processes of atmospheric uptake of CO₂ and its conversion into carbohydrate between mesophyll and bundle-sheath cells. As a result, most of the enzymes participating in the Calvin-Benson-Bassham (CBB) cycle, including RubisCO, are highly expressed in bundle-sheath cells. There is evidence that changes in the regulatory sequences of RubisCO contribute to its bundle-sheath-specific expression, however, little is known about how the spatial-expression pattern of other CBB cycle enzymes is regulated. In this study, we use a computational approach to scan for transcription factor binding sites in the regulatory regions of the genes encoding CBB cycle enzymes, SBPase, FBPase, PRK, and GAPDH-B, of C₃ and C₄ grasses. We identified potential cis-regulatory elements present in each of the genes studied here, regardless of the photosynthetic path used by the plant. The trans-acting factors that bind these elements have been validated in A. thaliana and might regulate the expression of the genes encoding CBB cycle enzymes. In addition, we also found C₄-specific transcription factor binding sites in the genes encoding CBB cycle enzymes that could potentially contribute to the pathway-specific regulation of gene expression. These results provide a foundation for the functional analysis of the differences in regulation of genes encoding CBB cycle enzymes between C₃ and C₄ grasses.

C ₄光合作用的特征是在大气中吸收CO ₂及其在叶肉细胞和束鞘细胞之间转化为碳水化合物的过程的区室化。结果，参与Calvin-Benson-Bassham（CBB）循环的大多数酶（包括RubisCO）在束鞘细胞中高度表达。有证据表明，调节序列的改变鲁比斯有助于其鞘鞘特异性表达，然而，关于如何调节其他CBB循环酶的空间表达模式知之甚少。在这项研究中，我们使用一种计算方法来扫描C ₃和C ₄草的编码CBB循环酶，SBPase，FBPase，PRK和GAPDH-B的基因的调节区域中的转录因子结合位点。我们确定了潜力顺式在这里研究的每个基因中都存在调控因子，而与植物使用的光合作用路径无关。结合这些元素的反式作用因子已在拟南芥并可能调节编码CBB循环酶的基因的表达。此外，我们还在编码CBB循环酶的基因中发现了C ₄特异性转录因子结合位点，这可能有助于基因表达的途径特异性调节。这些结果为功能分析C ₃和C ₄草之间编码CBB循环酶的基因差异提供了基础。