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The Response of Salinity Stress-Induced A. tricolor to Growth, Anatomy, Physiology, Non-Enzymatic and Enzymatic Antioxidants
Frontiers in Plant Science ( IF 5.6 ) Pub Date : 2020-08-17 , DOI: 10.3389/fpls.2020.559876
Umakanta Sarker 1 , Shinya Oba 2
Affiliation  

An investigation was carried out to elucidate growth, anatomical, physiological, and major ROS detoxification pathways involved in the tolerance of A. tricolor under salinity stress. Both VA14 and VA3 varieties exhibited the reduction in relative water content (RWC), photosynthetic pigments, growth, increased electrolyte leakage (EL), and leaf anatomy adaptation under salinity stress, whereas VA14 was well adapted and performed better compared to VA3. Higher ROS accumulation was demonstrated in the sensitive variety (VA3) in comparison to the tolerant variety (VA14). Salinity stress changed the cellular antioxidant pool by increasing total carotenoids, ascorbate, proline, total polyphenol content (TPC), total flavonoid content (TFC), and total antioxidant capacity (TAC) in both varieties. Although a higher increment was demonstrated in the tolerant variety, the proline increment was much more pronounced in the sensitive variety. Non-enzymatic antioxidant, ascorbate, carotenoids, TPC, TFC, TAC, and antioxidant enzymes SOD and APX were noted to be a major H2O2 detoxifier in the tolerant A. tricolor variety, where there is a comparatively lower H2O2 load. It was complemented by GPOX and CAT activity at a comparatively higher H2O2 load (in the sensitive variety). SOD contributed to the dismutation of superoxide radical (SOR) both in the tolerant and sensitive varieties; however, it greatly contributed to the dismutation of SOR in the tolerant variety. The increase in SOD, ascorbate, and APX makes it predominantly evident that SOD and the AsA–GSH cycle had greatly contributed to quench reactive oxygen species (ROS) of the tolerant variety of A. tricolor.



中文翻译:

盐度胁迫诱导的三色曲霉对生长、解剖、生理、非酶和酶抗氧化剂的反应

进行了一项研究,以阐明与耐受性相关的生长、解剖、生理和主要 ROS 解毒途径。A. 三色在盐胁迫下。VA14 和 VA3 品种在盐胁迫下均表现出相对含水量 (RWC)、光合色素、生长、电解质渗漏 (EL) 增加和叶片解剖适应的降低,而 VA14 适应良好,与 VA3 相比表现更好。与耐受品种 (VA14) 相比,敏感品种 (VA3) 具有更高的 ROS 积累。盐胁迫通过增加两个品种的总类胡萝卜素、抗坏血酸、脯氨酸、总多酚含量(TPC)、总黄酮含量(TFC)和总抗氧化能力(TAC)来改变细胞抗氧化剂库。尽管在耐受品种中表现出较高的增量,但在敏感品种中脯氨酸增量更为明显。非酶抗氧化剂、抗坏血酸、类胡萝卜素、TPC、TFC、TAC 和抗氧化酶 SOD 和 APX 被认为是耐受性中主要的 H 2 O 2解毒剂。A. 三色H 2 O 2负荷相对较低的品种。它在相对较高的 H 2 O 2负荷下(在敏感品种中)得到了 GPOX 和 CAT 活性的补充。SOD 有助于耐受和敏感品种中超氧自由基(SOR)的歧化;然而,它极大地促进了耐受品种中SOR的歧化。SOD、抗坏血酸和 APX 的增加表明,SOD 和 AsA-GSH 循环极大地促进了耐性品种的活性氧 (ROS) 的猝灭。A. 三色

更新日期:2020-10-17
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