Long non-coding RNAs (lncRNAs) emerge as critical regulators across a wide variety of biological functions in living organisms. However, to date, no systematic characterization of lncRNAs has been investigated in the ectoparasitic mite Varroa destructor, the most severe biotic threat to honey bees worldwide. Here, we performed an initial genome-wide identification of lncRNAs in V. destructor via high-throughput sequencing technology and reported, for the first time, the transcriptomic landscape of lncRNAs in the devastating parasite. By means of a lncRNA identification pipeline, 6,645 novel lncRNA transcripts, encoded by 3,897 gene loci, were identified, including 2,066 sense lncRNAs, 2,772 lincRNAs, and 1,807 lncNATs. Compared with protein-coding mRNAs, V. destructor lncRNAs are shorter in terms of full length, as well as of the ORF length, contain less exons, and express at lower level. GO term and KEGG pathway enrichment analyses of the lncRNA target genes demonstrated that these predicted lncRNAs may be potentially responsible for the regulatory functions of cellular and biological progresses in the reproductive phase of V. destructor. To our knowledge, this is the first catalog of lncRNA profile in the parasitiformes species, providing a valuable resource for genetic and genomic studies. Understanding the characteristics and features of lncRNAs in V. destructor would promote sustainable parasite control.

长链非编码 RNA (lncRNA) 成为生物体中多种生物功能的关键调节因子。然而，迄今为止，尚未对体外寄生螨中的 lncRNA 进行系统表征瓦螨，对全世界蜜蜂来说最严重的生物威胁。在这里，我们对 lncRNA 进行了初步全基因组鉴定五、析构函数通过高通量测序技术，首次报道了这种毁灭性寄生虫中 lncRNA 的转录组图谱。通过lncRNA鉴定流程，鉴定了由3,897个基因位点编码的6,645个新的lncRNA转录本，其中包括2,066个正义lncRNA、2,772个lincRNA和1,807个lncNAT。与编码蛋白质的mRNA相比，五、析构函数lncRNA的全长和ORF长度较短，包含较少的外显子，并且表达水平较低。 lncRNA靶基因的GO term和KEGG通路富集分析表明，这些预测的lncRNA可能潜在地负责生殖阶段细胞和生物学进展的调节功能。五、析构函数。据我们所知，这是寄生形目物种中第一个 lncRNA 谱目录，为遗传和基因组研究提供了宝贵的资源。了解lncRNA的特性和特点五、析构函数将促进可持续的寄生虫控制。