Abstract Introduction Due to the widespread occurrence of acrylamide in the environment, its likely carcinogen status, and the suitability of the pig model as a human analogue, the authors decided to evaluate the impact of high and low doses of this compound on the processes of erythropoiesis in swine bone marrow. Material and Methods The experiment was carried out on Danish Landrace pigs at the age of eight weeks and body weight about 20 kg. The animals were divided into three equal groups consisting of five pigs in each. Control animals received empty gelatin capsules (placebos). Animals from the first experimental group received a low dose of acrylamide of 0.5 μg/kg b.w./day (> 99% purity; Sigma-Aldrich, Poland), and animals from the second experimental group received a dose 10 times higher. Placebos and acrylamide capsules were administered with feed every morning for 28 days. After anaesthetisation of the animals, bone marrow from the femur was collected into tubes without an anticoagulant on days 0 and 28. After drying and staining, bone marrow smears were subjected to detailed cytological evaluation using a light microscope. Results This study showed that high and low doses of acrylamide affected the process of porcine erythropoiesis. The cytotoxic effect of acrylamide on this process was demonstrated in a change of the polychromatic erythroblasts/normochromatic erythroblasts ratio. Conclusion Both doses of acrylamide caused a decrease in the number of ortho- and polychromatic erythroblasts.

中文翻译：

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高、低剂量丙烯酰胺对猪红细胞生成的影响

摘要 引言由于丙烯酰胺在环境中的广泛存在、其可能的致癌物状态以及猪模型作为人体类似物的适用性，作者决定评估高剂量和低剂量这种化合物对红细胞生成过程的影响在猪骨髓中。材料与方法本实验在8周龄、体重约20公斤的丹麦长白猪身上进行。这些动物被分成三个相等的组，每组由五头猪组成。对照动物接受空明胶胶囊（安慰剂）。第一个实验组的动物接受 0.5 μg/kg bw/天的低剂量丙烯酰胺（> 99% 纯度；Sigma-Aldrich，波兰），而第二个实验组的动物接受的剂量高 10 倍。安慰剂和丙烯酰胺胶囊每天早上与饲料一起服用，持续 28 天。麻醉动物后，在第 0 天和第 28 天将股骨的骨髓收集到没有抗凝剂的试管中。干燥和染色后，使用光学显微镜对骨髓涂片进行详细的细胞学评估。结果本研究表明高、低剂量丙烯酰胺对猪红细胞生成过程的影响。丙烯酰胺对该过程的细胞毒性作用通过改变多色成红细胞/正色成红细胞比率来证明。结论 两种剂量的丙烯酰胺均导致原色和多色成红细胞数量减少。在第 0 天和第 28 天，将来自股骨的骨髓收集到没有抗凝剂的试管中。干燥和染色后，使用光学显微镜对骨髓涂片进行详细的细胞学评估。结果本研究表明高、低剂量丙烯酰胺对猪红细胞生成过程的影响。丙烯酰胺对该过程的细胞毒性作用通过改变多色成红细胞/正色成红细胞比率来证明。结论 两种剂量的丙烯酰胺均导致原色和多色成红细胞数量减少。在第 0 天和第 28 天，将来自股骨的骨髓收集到没有抗凝剂的试管中。干燥和染色后，使用光学显微镜对骨髓涂片进行详细的细胞学评估。结果本研究表明高、低剂量丙烯酰胺对猪红细胞生成过程的影响。丙烯酰胺对该过程的细胞毒性作用通过改变多色成红细胞/正色成红细胞比率来证明。结论 两种剂量的丙烯酰胺均导致原色和多色成红细胞数量减少。丙烯酰胺对该过程的细胞毒性作用通过改变多色成红细胞/正色成红细胞比率来证明。结论 两种剂量的丙烯酰胺均导致原色和多色成红细胞数量减少。丙烯酰胺对该过程的细胞毒性作用通过改变多色成红细胞/正色成红细胞比率来证明。结论 两种剂量的丙烯酰胺均导致原色和多色成红细胞数量减少。