Tea leafhopper (Empoasca onukii Matsuda) is amongst the key pests in tea plantations around the East Asian region. Stereomicroscopy is a conventional method used for detecting tea leafhopper eggs by dissecting the tender tissues. However, there is a need for a faster and more efficient method to directly observe and investigate intact eggs within tea shoots. The absence of a proven method limits research efforts for determining the oviposition behavior of E. onukii. Herein, we applied the blue light detection method (BLDM), a technique recently developed for other species, in order to detect E. onukii eggs directly and non-destructively within the tender shoot. In addition, we compared BLDM against the traditional stereomicroscope detection method (SMDM) for four tea cultivars. Notably, our results revealed that BLDM was precise and effective in measuring the egg laying quantity of E. onukii on intact tea shoots. Neither tea cultivars nor egg density in the tender shoot significantly affected the accuracy of BLDM. Furthermore, biological characteristics that have rarely been reported previously for E. onukii were investigated using the BLDM, including zygote duration, ovipositional rhythm, egg distribution within the tender shoot, and in different leaf positions, numbers of eggs laid by a single female daily, and laid by the entire generation. Therefore, these findings provide insights into the basic and theoretical evidence for the strategy and mechanism associated with the oviposition behavior of E. onukii.

中文翻译：

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蓝光检测法鉴定与蝉叶蝉叶卵产卵行为相关的生物学特性

茶蝉（Empoasca onukii Matsuda）是东亚地区茶园的主要害虫之一。立体镜检查是用于通过解剖嫩组织来检测叶蝉卵的常规方法。但是，需要一种更快，更有效的方法来直接观察和研究茶芽中的完整卵。缺乏可靠的方法限制了确定大肠埃希氏菌产卵行为的研究工作。在本文中，我们应用了最近针对其他物种开发的技术蓝光检测方法（BLDM），以检测大肠埃希氏菌。嫩芽内直接和非破坏性地产卵。此外，我们将BLDM与四种茶树种的传统立体显微镜检测方法（SMDM）进行了比较。值得注意的是，我们的结果表明，BLDM在测量大肠埃希氏菌产卵量方面是精确而有效的在完整的茶芽上。嫩芽中的茶品种和卵密度均未显着影响BLDM的准确性。此外，使用BLDM可以调查以前很少报道过的大肠埃希氏菌的生物学特性，包括合子持续时间，产卵节律，嫩枝内卵的分布以及在不同叶片位置，单只雌性每天产卵的数量，并为整个世代奠定。因此，这些发现为与大肠埃希氏菌产卵行为相关的策略和机制提供了基础和理论证据。