The cobblestone tiger beetle, Cicindelidia marginipennis (Dejean, 1831) is a North American species specializing in riparian habitats from New Brunswick, Canada, to Alabama in the United States. In the United States, this species is state-listed as threatened or endangered range-wide and periodically receives consideration for federal listing, mostly due to habitat decline. Despite its conservation status, intraspecific genetic diversity for this species has not been explored and little is known about its natural history. To support further inquiry into the biology of C. marginipennis, this study provides the first look at range-wide genetic diversity using mitochondrial DNA (mtDNA), describes all three larval instars, and describes natural history characteristics from captive rearing and field observation. Based on mtDNA analyses, our results suggest that geographically based population structure may exist throughout the range, with individuals from Alabama possessing haplotypes not found elsewhere in our sampling. Further genetic analyses, particularly multi-locus analyses, are needed to determine whether the Alabama population represents a separate cryptic species. Our morphological analysis and descriptions of larval instars reveal a combination of characteristics that can be used to differentiate C. marginipennis from closely related and co-occurring species. Based on our field observations, we find that the larval “throw pile” of soil excavated from burrows is a key search image for locating larvae, and we provide descriptions and detailed photographs to aid surveys. Lastly, we find that this species can be successfully reared in captivity and provide guidelines to aid future recovery efforts.

中文翻译：

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濒危鹅卵石老虎甲虫的系统文字变化，幼虫特征和实验室饲养的第一个记录，德吉恩，Cicindelidia marginipennis，1831年，并观察了它们的自然历史

鹅卵石老虎甲虫Cicindelidia marginipennis（Dejean，1831年）是北美物种，专门研究从加拿大新不伦瑞克省到美国阿拉巴马州的河岸生境。在美国，该物种被列为全范围受威胁或濒危物种，并定期受到联邦政府的考虑，这主要是由于栖息地减少所致。尽管具有该物种的保护地位，但尚未对该物种的种内遗传多样性进行探索，对其自然历史知之甚少。支持进一步研究毛缘衣原体的生物学，这项研究首次使用线粒体DNA（mtDNA）研究了全范围遗传多样性，描述了所有三只幼虫，并描述了圈养和田间观察的自然史特征。根据mtDNA分析，我们的结果表明，基于地理的种群结构可能在整个范围内都存在，来自阿拉巴马州的个体具有在我们的抽样中未发现的单倍型。需要进一步的遗传分析，尤其是多基因座分析，以确定阿拉巴马州的种群是否代表一个单独的隐性物种。我们对幼虫期幼虫的形态学分析和描述揭示了可用于区分边缘隐孢子虫的特征的组合来自密切相关且同时存在的物种。根据我们的现场观察，我们发现从洞穴挖出的土壤的幼虫“扔堆”是定位幼虫的关键搜索图像，并且我们提供了描述和详细的照片以协助调查。最后，我们发现可以成功地圈养该物种并提供指导以帮助将来的恢复工作。