miRNA is a small non-coding RNA, which plays an important role in diverse biological processes. In the present study, we explore the effect of ssc-miR-451 on porcine adipose development and meat quality. We observed that ssc-miR-451 was downregulated during porcine primary adipocyte differentiation. Overexpression of ssc-miR-451 inhibited adipogenic differentiation, while inhibition of ssc-miR-451 promoted adipogenic differentiation. The dual luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as a target gene of ssc-miR-451. Correlation analysis negatively correlated miR-451 expression with intramuscular fat content (IMF) and positively correlated ACACA expression with IMF. Further analysis of fatty acid composition revealed that pigs with high expression of ssc-miR-451 had higher monounsaturated fatty acid (MUFA) and lower polyunsaturated fatty acid (PUFA). Taken together, our study suggests that ssc-miR-451 regulates lipid deposition and fatty acid composition by targeting ACACA, and ssc-miR-451 may serve as a potential genetic marker to improve pork quality.

中文翻译：

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ssc-miR-451通过靶向ACACA调节猪原代脂肪细胞分化

miRNA是小的非编码RNA，在各种生物学过程中都起着重要作用。在本研究中，我们探讨了ssc-miR-451对猪脂肪发育和肉质的影响。我们观察到猪原发性脂肪细胞分化过程中ssc-miR-451被下调。ssc-miR-451的过表达抑制了成脂分化，而ssc-miR-451的抑制则促进了成脂分化。双重荧光素酶报告系统表明乙酰辅酶A羧化酶α（ACACA）是ssc-miR-451的靶基因。相关分析表明miR-451表达与肌内脂肪含量（IMF）呈负相关，而ACACA表达与IMF呈正相关。脂肪酸组成的进一步分析表明，高表达ssc-miR-451的猪具有较高的单不饱和脂肪酸（MUFA）和较低的多不饱和脂肪酸（PUFA）。综上所述，我们的研究表明ssc-miR-451通过靶向ACACA来调节脂质沉积和脂肪酸组成，而ssc-miR-451可能是改善猪肉品质的潜在遗传标记。