New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co²⁺ ions by patterned UV light activates Terminal deoxynucleotidyl Transferase (TdT) for spatially-selective synthesis on an array surface. Spontaneous quenching of reactions by the diffusion of excess caging molecules confines synthesis to light patterns and controls the extension length. We show that our multiplexed synthesis method can be used to store digital data by encoding 12 unique DNA oligonucleotide sequences with video game music, which is equivalent to 84 trits or 110 bits of data.

需要新的存储技术来满足全球数据生成的需求。 DNA 因其稳定性、信息密度和易于通过先进测序技术读出而成为理想的存储介质。然而，对化学合成方法的持续依赖阻碍了 DNA 写入的进展。 DNA 的酶促合成是一种很有前途的替代方案，但迄今为止尚未以并行方式得到很好的证明。在这里，我们报告了一种使用无掩模光刻的多重酶促 DNA 合成方法。通过图案化紫外光快速释放 Co ²⁺离子可激活末端脱氧核苷酸转移酶 (TdT)，从而在阵列表面进行空间选择性合成。通过过量笼养分子的扩散来自发猝灭反应，将合成限制在光模式并控制延伸长度。我们证明，我们的多重合成方法可以通过用视频游戏音乐编码 12 个独特的 DNA 寡核苷酸序列来存储数字数据，相当于 84 个 trit 或 110 位数据。