Abstract

Three-dimensional (3 D) printing is an effective technology that has shown considerable potential for use in tissue regeneration. Of the many materials that have been proposed for this purpose, poly (ε-caprolactone) (PCL) 3 D scaffolds have been received significant attention in the bone tissue engineering field due to its advantageous mechanical properties and biocompatibility. In this study, a novel method was developed for tissue-engineered bone that combines PCL 3 D scaffolds with fish collagen (Col) and the osteogenic abalone intestine gastro-intestinal digests (AIGIDs) from Haliotis discus hannai. And then, mouse mesenchymal stem cells (MSCs) were seeded onto the fabricated scaffolds. After in vitro culturing, the proliferation of the MSCs on the scaffolds, alkaline phosphatase (ALP) activity, and the amount of deposited calcium were investigated. The results indicated that the ALP activity and mineralization in PCL/AIGIDs/Col was higher than that of the other scaffolds. In an in vivo experiment, the two fabricated scaffolds were implanted in a rabbit tibia. PCL/AIGIDs/Col group exhibited strong osteoinduction capability in the rabbit tibia defect model. These stimulated biological responses in vitro and in vivo suggest that the PCL/AIGIDs/Col scaffold are promising material for use in tissue implants and bone regeneration.

摘要

三维 (3D) 打印是一种有效的技术，已在组织再生中显示出巨大的潜力。在为此目的而提出的许多材料中，聚（ε-己内酯）（PCL）3D 支架由于其优越的机械性能和生物相容性而在骨组织工程领域受到了极大的关注。在这项研究中，开发了一种用于组织工程骨的新方法，该方法将 PCL 3D 支架与鱼胶原蛋白 (Col) 和来自Haliotis discus hannai的成骨鲍鱼肠胃肠消化物 (AIGID) 相结合。然后，将小鼠间充质干细胞 (MSC) 接种到制造的支架上。后在体外培养、支架上 MSC 的增殖、碱性磷酸酶 (ALP) 活性和沉积的钙量进行了研究。结果表明PCL/AIGIDs/Col中的ALP活性和矿化高于其他支架。在体内实验中，将两个制作好的支架植入兔胫骨。PCL/AIGIDs/Col组在兔胫骨缺损模型中表现出较强的骨诱导能力。这些体外和体内刺激的生物反应表明 PCL/AIGIDs/Col 支架是用于组织植入和骨再生的有前途的材料。