Abstract

The genus Daphne presents great interest as a valuable source of natural agents in traditional medicine of various countries, including Turkey. In the current work, we investigated the phytochemical composition and biological properties of Daphne pontica L. extracts. Methanol extracts of roots, stems and leaves were prepared. The chemical characterization was performed by HPLC-ESI-MSⁿ, observing that flavonoids and phenolic acids were the main compounds. Biological properties were studied in terms of antimicrobial, DNA protection, anticancer, and enzyme inhibitory properties (cholinesterases, tyrosinase, amylase and glucosidase). Methanol extracts of roots, leaves, and stems of D. pontica had antimicrobial effects against a broad range of bacteria. In addition, methanol extract of roots of D. pontica exhibited a promising anticancer activity that was shown to be dose- and time-dependent for HeLa cells with an IC₅₀ value of 203.9 μg/mL. Generally, the leaf extracts exhibited the best enzyme inhibitory properties on the tested enzymes. According to our results, D. pontica might be regarded as a valuable source of natural agents to combat cancer, infectious diseases, and metabolic diseases .

摘要

达芙妮属在包括土耳其在内的各国传统医学中作为天然药物的宝贵来源表现出极大的兴趣。在目前的工作中，我们研究了瑞香提取物的植物化学成分和生物学特性。制备根、茎和叶的甲醇提取物。通过 HPLC-ESI-MS ⁿ进行化学表征，观察到黄酮类化合物和酚酸是主要化合物。在抗菌、DNA 保护、抗癌和酶抑制特性（胆碱酯酶、酪氨酸酶、淀粉酶和葡萄糖苷酶）方面研究了生物学特性。D. pontica根、叶和茎的甲醇提取物对多种细菌具有抗菌作用。此外，D. pontica根的甲醇提取物表现出有希望的抗癌活性，其对 HeLa 细胞具有剂量和时间依赖性，IC ₅₀值为 203.9 μg/mL。一般来说，叶子提取物对测试的酶表现出最好的酶抑制特性。根据我们的研究结果，D. pontica可能被视为对抗癌症、传染病和代谢疾病的天然药物的宝贵来源。