ABSTRACT

The effects of flavonoids, in the medical formulation Daflon 500 mg (DAF) in protection of the heart against ischemia/reperfusion are not completely understood. Heart function data from Langendorff perfused Wistar rat hearts (n = 8/group) were digitally computed, and the infarct size was evaluated using 2,3,5-triphenyl tetrazolium chloride (TTC) staining and cardiac enzyme levels. The total oxidants (TOS) and antioxidants (TAS) levels as well as pro- and anti-inflammatory cytokines levels were estimated by TOS and TAS assay kits and enzyme-linked immunosorbent assay (ELISA), respectively. The antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were evaluated using RANDOX kits. Longtime treatment of DAF for 2 or 4 weeks normalized cardiac hemodynamics, vascular dynamics, and heart contractility. A significant reduction in the infarct size and cardiac enzyme levels (P < .05) was produced by DAF treatment. Short time infusion of DAF two hours before inducing ischemia yielded similar results. DAF treatment significantly (P < .05) decreased TOS levels in the perfusion effluent and increased SOD levels in the cardiomyocytes. In conclusion, treatment with DAF prevents the deterioration of cardiac function and ischemic injury caused by oxidative stress and inflammatory cytokines. The protective effects of DAF are possibly due to its antioxidant and anti-inflammatory properties.

摘要

医学制剂Daflon 500 mg（DAF）中的类黄酮在保护心脏免于缺血/再灌注方面的作用尚不完全清楚。来自Langendorff灌注Wistar大鼠心脏的心脏功能数据（n= 8 /组），并用2,3,5-三苯基氯化四氮唑（TTC）染色和心肌酶水平评估梗塞面积。总氧化剂（TOS）和抗氧化剂（TAS）的水平以及促炎和抗炎细胞因子的水平分别通过TOS和TAS测定试剂盒和酶联免疫吸附测定（ELISA）进行估算。使用RANDOX试剂盒评估了抗氧化酶，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。DAF的2或4周的长期治疗可使心脏血液动力学，血管动力学和心脏收缩力正常化。通过DAF治疗可显着减少梗塞面积和心肌酶水平（P <.05）。在诱导缺血前两个小时短时间输注DAF可获得相似的结果。DAF治疗显着（P <.05）降低了灌注液中的TOS水平，并增加了心肌细胞中的SOD水平。总之，DAF的治疗可防止由氧化应激和炎性细胞因子引起的心脏功能恶化和缺血性损伤。DAF的保护作用可能是由于其抗氧化和抗炎特性。