ABSTRACT

Soil-borne Fusarium wilt disease causes damage to certain crops, but crop rotation may be an effective management strategy for this pest. Soil microbes may play an important role in this strategy, but the response of the soil microbial community to crop rotation is unclear. In a field experiment, we used high-throughput sequencing to study the effects of banana rotation with one of four other crops (pepper, sugarcane, wax gourd and pumpkin) or continuous cultivation of banana as a single crop on soil microbial community. Compared with monocropping, all crop rotation strategies led to a decrease in the incidence of Fusarium wilt of banana, while an increase in banana yields. The main bacterial phyla were Proteobacteria (24.96%), Acidobacteria (21.25%), Gemmatimonadetes (12.98%), and the fungal phyla was Ascomycota (72.54%), across the soil. These four crop rotation treatments also increased bacterial richness and the relative abundance of Acidobacteria, Gemmatimonadetes, Firmicutes, and Actinobacteria, while reducing the relative abundance of Proteobacteria, Chloroflexi, Parcubacteria, Cyanobacteria and Fusarium. Redundancy analysis showed that soil pH, organic matter and available phosphorus were the main factors affecting the composition of bacterial and fungal communities. Overall, we show that crop rotation can inhibit banana wilt and change soil microbial community composition.

摘要

土壤传播的枯萎病会对某些作物造成损害，但轮作可能是这种害虫的有效管理策略。土壤微生物可能在这一策略中发挥重要作用，但土壤微生物群落对轮作的反应尚不清楚。在田间试验中，我们使用高通量测序来研究香蕉与其他四种作物（辣椒、甘蔗、冬瓜和南瓜）中的一种轮作或连续种植香蕉作为单一作物对土壤微生物群落的影响。与单作相比，所有轮作策略均导致香蕉枯萎病发生率降低，而香蕉产量增加。主要细菌门是变形菌门（24.96%）、酸杆菌门（21.25%）、Gemmatimonadetes（12.98%），真菌门是子囊菌门（72.54%），分布在整个土壤中。镰刀菌。冗余分析表明，土壤pH、有机质和速效磷是影响细菌和真菌群落组成的主要因素。总体而言，我们表明轮作可以抑制香蕉枯萎病并改变土壤微生物群落组成。