Abstract

The present study was aimed to isolate active constituents from Blumea axillaris (Lam.) DC (Asteraceae) against phytopathogenic fungi. Bioactivity guided fractionation of the successive n-hexane, chloroform and methanol extract led to the isolation of the monoterpene ester (4 R,5S)-4-hydroxy-7-tigloyloxycarvotanacetone (1). The compound 1 was converted into acetyl derivative (2). The acetyl derivative (2) and the parent compound 1 were tested again phytopathogenic fungi by using mycelial inhibition and minimal inhibitory concentration values were found out by the broth microdilution method. The acetyl derivative (2) showed the highest antifungal activity against Rhizoctonia solani and Aspergillus niger. Based upon in vitro results, compound 1 was tested against Fusarium oxyporum (wilting disease) and compound 2 was tested against R. solani (leaf blight disease) in vivo using the foliar spray method. Both compounds had no phytotoxicity and also in silico docking study showed that both compounds were binding similarly as commercial fungicide carbendazim.

摘要

本研究旨在从Blumea axillaris (Lam.) DC (Asteraceae) 中分离抗植物病原真菌的活性成分。连续的生物活性引导分级Ñ导致了单萜酯（4的隔离-己烷，氯仿和甲醇提取物 - [R，5小号）-4-羟基-7- tigloyloxycarvotanacetone（1）。将化合物1转化为乙酰基衍生物(2)。乙酰基衍生物(2)和母体化合物1用菌丝抑制法再次检测植物病原真菌，用肉汤微量稀释法求出最小抑菌浓度值。乙酰基衍生物 (2) 对立枯丝核菌的抗真菌活性最高，黑曲霉。基于体外结果，使用叶面喷洒法在体内测试了化合物 1 对抗枯萎病（枯萎病）和化合物 2 对抗立枯病菌（叶枯病）。两种化合物都没有植物毒性，并且在计算机对接研究中也表明两种化合物的结合与商业杀菌剂多菌灵相似。