Abstract

The purpose of this research was to evaluate the efficacy of carsil (CAR) either alone or in combination with α-tocopherol (α-TOCO) and/or turmeric (TUMR) against tetrachloromethane (TCM)-induced cardiomyocyte injury in rats. Administration of CAR either alone or in combination with α-TOCO and/or TUMR post-TCM injection, significantly mitigated the increases in serum troponin T, creatine kinase-MB (CK-MB) as well as interleukin-6 (IL-6), interferon γ (IFN-γ), tumor necrosis factor-α (TNF-α), C-reactive protein (CRP). They also decline the elevation of caspase-3, vascular endothelial growth factor (VEGF) protein expression as well as DNA damage in cardiac tissues induced by TCM. The biochemical results were confirmed by histopathological investigation. Conclusion: The combination of the three antioxidants showed greater cardioprotective potential, compared to individual drugs. Therefore, this combination may be recommended as a complementary therapy to antagonize cardiac injury induced by different insults.

摘要

本研究的目的是评估 carsil (CAR) 单独或与 α-生育酚 (α-TOCO) 和/或姜黄 (TUMR) 联合使用对四氯甲烷 (TCM) 诱导的大鼠心肌细胞损伤的疗效。CAR 单独给药或与 α-TOCO 和/或 TUMR 联合给药后 TCM 注射，显着减轻血清肌钙蛋白 T、肌酸激酶-MB (CK-MB) 以及白细胞介素-6 (IL-6) 的增加、干扰素γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、C反应蛋白（CRP）。它们还降低了由中药诱导的心脏组织中 caspase-3、血管内皮生长因子 (VEGF) 蛋白表达以及 DNA 损伤的升高。生化结果经组织病理学检查证实。结论：三种抗氧化剂的组合显示出更大的心脏保护潜力，与个别药物相比。因此，可以推荐这种组合作为补充疗法来对抗由不同损伤引起的心脏损伤。