This study aims at determining the in vitro antitrypanosomal, antileishmanial, antioxidant, and anti-inflammatory-like activities of Terminalia mollis root crude extracts. The antitrypanosomal and antileishmanial activities on Trypanosoma brucei brucei (strain 427) and promastigotes of Leishmania mexicana mexicana (MHOM/BZ/84/BEL46) were evaluated in vitro. The methanolic root bark extract and standards were profiled by HPLC-PDA, and the majority of compounds identified using literature data. The in vitro antioxidant and anti-inflammatory-like activities were determined by evaluating the effect of crude extracts on reactive oxygen species produced by phorbol 12-myristate 13-acetate-stimulated equine neutrophils using lucigenin-enhanced chemiluminescence and on purified equine myeloperoxidase activity measured by specific immunological extraction followed by enzymatic detection. The methanolic, aqueous crude extract, and aqueous crude extract free of tannins exhibited good growth inhibition on Trypanosoma brucei brucei (IC50 3.72, 6.05, and 4.45 µg/mL respectively) but were inactive against Leishmania mexicana mexicana (IC50 > 100 µg/mL). Suramin (IC50 0.11 µg/mL) and amphotericin (IC50 0.11 µg/mL) were used as standard respectively for the antitrypanosomal and antileishmanial activity. Very interesting antioxidant and anti-inflammatory-like activities were observed with 50% hydroethanolic, aqueous crude extracts, and aqueous crude extract free of tannins as well as with pure punicalagin, gallic, and ellagic acid (IC50 0.38 - 10.51 µg/mL for 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), chemiluminescence, and specific immunological extraction followed by enzymatic detection assays. The study results support traditional medicinal use of the plant for the treatment of parasitical disorders and revealed for the first time the antitrypanosomal potential, anti-inflammatory-like, and antioxidant activity of Terminalia mollis root.

中文翻译：

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榄仁树根皮的体外抗利什曼原虫、抗锥虫病和抗炎样活性

本研究旨在确定榄仁树根粗提取物的体外抗锥虫、抗利什曼原虫、抗氧化和抗炎样活性。在体外评估了对布氏锥虫（菌株 427）和墨西哥利什曼原虫（MHOM/BZ/84/BEL46）的前鞭毛体的抗锥虫和抗利什曼原虫活性。甲醇根皮提取物和标准品通过 HPLC-PDA 进行分析，大多数化合物使用文献数据进行鉴定。通过使用光泽精增强的化学发光评估粗提物对佛波醇 12-肉豆蔻酸酯 13-乙酸酯刺激的马中性粒细胞产生的活性氧的影响，以及对纯化马髓过氧化物酶活性的影响，测定体外抗氧化和抗炎活性。特异性免疫提取，酶法检测。甲醇、粗提物和不含单宁的粗提物对布氏锥虫表现出良好的生长抑制作用（IC50 分别为 3.72、6.05 和 4.45 µg/mL），但对墨西哥利什曼原虫无活性（IC50 > 100 µg/mL） . 苏拉明 (IC50 0.11 µg/mL) 和两性霉素 (IC50 0.11 µg/mL) 分别用作抗锥虫病和抗利什曼原虫活性的标准品。38 - 10.51 µg/mL 用于 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)、化学发光和特异性免疫提取，然后进行酶检测。研究结果支持该植物用于治疗寄生虫病的传统药用，并首次揭示了榄仁树根的抗锥虫潜力、抗炎样和抗氧化活性。38 - 10.51 µg/mL 用于 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)、化学发光和特异性免疫提取，然后进行酶检测。研究结果支持该植物用于治疗寄生虫病的传统药用，并首次揭示了榄仁树根的抗锥虫潜力、抗炎样和抗氧化活性。