This study intends to address the function of miR‐301b/nuclear receptor subfamily 3 group C member 2 (NR3C2) in breast cancer. The Cancer Genome Atlas database was processed to investigate the expression of miR‐301b/NR3C2 in breast cancer samples, as well as the relationship between their expression and the prognosis of the patients. Cox regression analysis was performed to determine whether miR‐301b/NR3C2 was an independent predictor of the patient's prognosis. Associations between miR‐301b and NR3C2 were analyzed by prediction website, dual‐luciferase assay, and Pearson correlation coefficient. Quantitative polymerase chain reaction and Western blot analyses were implemented to detect gene expression. The relevant biological characteristics of MCF7 and BCAP‐37 cells were tested by cell counting kit‐8, colony formation, and transwell assays. Lower expression of NR3C2, which was closely related to the bad prognosis of breast cancer patients, was presented in breast cancer samples and can be used as an independent predictor. miR‐301b, as an upstream regulator of NR3C2, was highly expressed in breast cancer samples and can be used as an independent predictor as well. Notably, a higher level of miR‐301b and lower level of NR3C2 were related to the reduced overall survival in patients with breast cancer. The proliferative and migratory behaviors of cells were elevated or blocked after overexpression of miR‐301b or NR3C2, respectively. However, the above situation was attenuated after together upregulation of miR‐301b and NR3C2. The present data afforded evidence that miR‐301b may be a tumor‐promoting miRNA in breast cancer, and that miR‐301b/NR3C2 axis mediated tumor development from cell proliferation and migration.

中文翻译：

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miR-301b 和 NR3C2 共同调节细胞的恶性特性，并有可能成为乳腺癌的独立预后因素


本研究旨在探讨 miR-301b/核受体亚家族 3 C 组成员 2 (NR3C2) 在乳腺癌中的功能。对癌症基因组图谱数据库进行处理，研究乳腺癌样本中 miR-301b/NR3C2 的表达及其表达与患者预后的关系。进行 Cox 回归分析以确定 miR-301b/NR3C2 是否是患者预后的独立预测因子。通过预测网站、双荧光素酶测定和 Pearson 相关系数分析 miR-301b 和 NR3C2 之间的关联。进行定量聚合酶链反应和蛋白质印迹分析来检测基因表达。通过细胞计数试剂盒8、集落形成和Transwell实验测试MCF7和BCAP-37细胞的相关生物学特性。 NR3C2的低表达与乳腺癌患者的不良预后密切相关，在乳腺癌样本中存在，可作为独立的预测因子。 miR-301b作为NR3C2的上游调节因子，在乳腺癌样本中高表达，也可以用作独立的预测因子。值得注意的是，较高水平的 miR-301b 和较低水平的 NR3C2 与乳腺癌患者总生存期降低有关。过表达 miR-301b 或 NR3C2 后，细胞的增殖和迁移行为分别得到增强或阻断。然而，miR-301b 和 NR3C2 共同上调后，上述情况减弱。目前的数据提供了证据，表明 miR-301b 可能是乳腺癌中的一种促肿瘤 miRNA，并且 miR-301b/NR3C2 轴介导细胞增殖和迁移的肿瘤发展。