Fungal endophytes from leaves of Avicennia marina growing in semi‐arid environment as a promising source for bioactive compounds,Letters in Applied Microbiology

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Fungal endophytes from leaves of Avicennia marina growing in semi‐arid environment as a promising source for bioactive compounds
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-11-18 , DOI: 10.1111/lam.13414
A.M.A. Khalil _{1,

2} , A.M. Abdelaziz ₁ , M.M. Khaleil _{2,

3} , A.H. Hashem ₁

Affiliation

Endophytic fungi are broadly dispersed residing inside plant tissues and have been demonstrated as a treasure for bioactive natural products. Unexplored harsh and heavy metal contaminant habitat of Avicennia marina may have diverse and potential fungal association. Therefore, this work aimed to isolate the culturable fungal endophytes associated with leaves of A. marina and to evaluate their medical potentialities. Seventeen isolates of endophyte fungi were isolated from healthy leaves and their antimicrobial activities were evaluated. Results showed that isolates had activity against microorganisms in addition to their antioxidant activity, produced a variety of phenolic compounds, besides exhibited a lowest cytotoxicity against ATCC-CCL-81cell line. Consequently, selected endophytic fungal isolates were identified genetically as Chaetomium sp., Chaetomium. madrasense, Chaetomium. sp, Chaetomium. globosum, Aspergillus hiratsukae, Aspergillus ochraceus, Alternaria tenuissima and Curvularia lunata with gene bank accession numbers MT089951, MT089952, MT089953, MT089954, MT089955, MT089956, MT089957 and MT089958 respectively. The most potent fungus extract was analyzed using Gas chromatography-mass spectrometry (GC-MS) which verified the presence of numerous bioactive compounds. These findings confirmed that new endophytic fungal strains derived from A. marina thrive in harsh ecosystem produce bioactive metabolites which can be recommended as a novel source for drug discovery.

中文翻译：

生长在半干旱环境中的 Avicennia marina 叶子的真菌内生菌是生物活性化合物的有希望的来源

内生真菌广泛分布于植物组织内，已被证明是具有生物活性的天然产物的宝藏。Avicennia marina 未开发的恶劣和重金属污染物栖息地可能与多种潜在的真菌关联。因此，这项工作旨在分离与 A. marina 叶子相关的可培养真菌内生菌并评估它们的医学潜力。从健康叶片中分离出 17 株内生真菌，并对它们的抗菌活性进行了评价。结果表明，分离株除了具有抗氧化活性外，还具有抗微生物活性，产生多种酚类化合物，并且对ATCC-CCL-81细胞系的细胞毒性最低。因此，选定的内生真菌分离株在遗传上被鉴定为毛壳菌属，毛壳菌。madrasense，毛壳菌。sp，毛壳菌属。globosum、Aspergillus hiratsukae、Aspergillus ochraceus、Alternaria tenuissima 和 Curvularia lunata，其基因库登录号分别为 MT089951、MT089952、MT089953、MT089954、MT089955、MT089595989MT。使用气相色谱-质谱法 (GC-MS) 分析了最有效的真菌提取物，这证实了许多生物活性化合物的存在。这些发现证实，源自 A. marina 的新内生真菌菌株在恶劣的生态系统中茁壮成长，产生的生物活性代谢物可推荐为药物发现的新来源。分别为 MT089954、MT089955、MT089956、MT089957 和 MT089958。使用气相色谱-质谱法 (GC-MS) 分析了最有效的真菌提取物，这证实了许多生物活性化合物的存在。这些发现证实，源自 A. marina 的新内生真菌菌株在恶劣的生态系统中茁壮成长，产生的生物活性代谢物可推荐为药物发现的新来源。分别为 MT089954、MT089955、MT089956、MT089957 和 MT089958。使用气相色谱-质谱法 (GC-MS) 分析了最有效的真菌提取物，这证实了许多生物活性化合物的存在。这些发现证实，源自 A. marina 的新内生真菌菌株在恶劣的生态系统中茁壮成长，产生的生物活性代谢物可推荐为药物发现的新来源。

更新日期：2020-11-18

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