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Antibacterial activity and synergy of antibiotics with sanguisorbigenin isolated from Sanguisorba officinalis L. against methicillin‐resistant Staphylococcus aureus
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-01-08 , DOI: 10.1111/lam.13417 S. Wang 1 , J. Luo 2 , X.‐Q. Liu 2 , O.‐H. Kang 1 , D.‐Y. Kwon 1
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-01-08 , DOI: 10.1111/lam.13417 S. Wang 1 , J. Luo 2 , X.‐Q. Liu 2 , O.‐H. Kang 1 , D.‐Y. Kwon 1
Affiliation
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The present study evaluated the antibacterial activity and the synergy of the sanguisorbigenin from the dried root of Sanguisorba officinalis L. combined with β-lactam antibiotics against methicillin-resistant Staphylococcus aureus. A total of six strains of reference strain and clinical isolates were used to determine the antibacterial activity using a broth microdilution assay, and the synergistic effects were determined using a checkerboard assay. To analyze the mechanism of synergy, we conducted the level of penicillin-binding protein 2a by western blot. In addition, quantitative RT-PCR was performed to analyze the mecA gene expression. The minimal inhibitory concentration values of sanguisorbigenin against six strains of S. aureus were in the range of 12.5-50 μg ml-1 , and there were synergy, or partial synergy effects when sanguisorbigenin was combined with antibiotics. Furthermore, when treated with sanguisorbigenin, the level of penicillin-binding protein 2a and the expression of the mecA gene was reduced significantly. In conclusion, this study demonstrated that sanguisorbigenin is a potential natural antibacterial agent against methicillin-resistant Staphylococcus aureus that represents a considerable burden on the healthcare system worldwide, and may an exceptionally modulator of β-lactam antibiotics.
中文翻译：
抗菌活性和抗生素与从 Sanguisorba officinalis L. 中分离的 sanguisorbigenin 对耐甲氧西林金黄色葡萄球菌的抗菌活性和协同作用
本研究评估了山梨木干根中的山梨木糖苷元联合β-内酰胺类抗生素对耐甲氧西林金黄色葡萄球菌的抗菌活性和协同作用。使用肉汤微量稀释法测定了总共六株参考菌株和临床分离株的抗菌活性，并使用棋盘法测定了协同效应。为了分析协同作用的机制，我们通过蛋白质印迹进行了青霉素结合蛋白 2a 的水平。此外，还进行了定量 RT-PCR 以分析 mecA 基因表达。血山梨醇苷元对六株金黄色葡萄球菌的最小抑菌浓度值在12.5-50 μg ml-1 范围内，存在协同作用，或与抗生素合用时有部分协同作用。此外，当用sanguisorbigenin处理时，青霉素结合蛋白2a的水平和mecA基因的表达显着降低。总之，这项研究表明，sanguisorbigenin 是一种潜在的天然抗菌剂，可以对抗耐甲氧西林金黄色葡萄球菌，这对全球医疗保健系统构成了相当大的负担，并且可能是 β-内酰胺抗生素的特殊调节剂。
更新日期：2021-01-08
中文翻译：
抗菌活性和抗生素与从 Sanguisorba officinalis L. 中分离的 sanguisorbigenin 对耐甲氧西林金黄色葡萄球菌的抗菌活性和协同作用
本研究评估了山梨木干根中的山梨木糖苷元联合β-内酰胺类抗生素对耐甲氧西林金黄色葡萄球菌的抗菌活性和协同作用。使用肉汤微量稀释法测定了总共六株参考菌株和临床分离株的抗菌活性，并使用棋盘法测定了协同效应。为了分析协同作用的机制，我们通过蛋白质印迹进行了青霉素结合蛋白 2a 的水平。此外，还进行了定量 RT-PCR 以分析 mecA 基因表达。血山梨醇苷元对六株金黄色葡萄球菌的最小抑菌浓度值在12.5-50 μg ml-1 范围内，存在协同作用，或与抗生素合用时有部分协同作用。此外，当用sanguisorbigenin处理时，青霉素结合蛋白2a的水平和mecA基因的表达显着降低。总之，这项研究表明，sanguisorbigenin 是一种潜在的天然抗菌剂，可以对抗耐甲氧西林金黄色葡萄球菌，这对全球医疗保健系统构成了相当大的负担，并且可能是 β-内酰胺抗生素的特殊调节剂。
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