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Cytochrome oxidase gene sequencing reveals channel catfish ovary cell line is contaminated with brown bullhead cells
Journal of Fish Diseases ( IF 2.5 ) Pub Date : 2020-10-17 , DOI: 10.1111/jfd.13278
Lorelei Ford 1 , Kuttichantran Subramaniam 2 , Thomas B. Waltzek 2 , Paul R. Bowser 3 , Larry Hanson 1
Affiliation  

The channel catfish (Ictalurus punctatus, Rafinesque) ovary (CCO) cell line is the standard cell line used for channel catfish diagnostics. Next‐gen sequencing studies of a virus cultured in the CCO cells revealed mitochondrial sequences matching those of brown bullhead (Ameiurus nebulosus, Lesueur). Therefore, we systematically performed partial cytochrome oxidase 1 gene sequencing of several sources of the CCO cell line and all matched the brown bullhead and not the channel catfish.

中文翻译:

细胞色素氧化酶基因测序发现channel鱼卵巢细胞系被棕色牛头细胞污染

channel鱼(Ictalurus punctatus, Rafinesque)卵巢(CCO)细胞系是用于channel鱼诊断的标准细胞系。对在CCO细胞中培养的病毒进行的下一代测序研究表明,线粒体序列与棕色牛头(Ameiurus nebulosus, Lesueur)的线粒体序列匹配。因此,我们系统地对CCO细胞系的几种来源进行了细胞色素氧化酶1基因的部分测序,并且全部与棕色bull头匹配,而不与channel鱼匹配。
更新日期:2020-12-10
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