Tamarix is an important genus of Tamarieaceae family, known for its medicinal importance. Its taxonomy is tricky, and a few molecular makers are available for species identification. The present study was designed to evaluate the genetic diversity among 21 Tamarix specimens collected from Thal desert, Punjab, Pakistan. Ten Inter-Simple Sequence Repeats (ISSR) and six Simple Sequence Repeats (SSR) markers were used. Results revealed that ISSR markers produced 131 bands, of which 116 were polymorphic (88.5%) with a mean Polymorphic Information Content (PIC) value of 0.34. The SSR markers yielded 30 amplified bands, of which 24 were polymorphic (80.28%) with mean PIC value of 0.35. Cluster and factorial analysis revealed a high level of genetic variability among tested Tamarix ecotypes. Dissimilarity index indicated a genetic variability among tested specimens ranging from 0.00 to 0.77. The ISSR analysis reflected that Tamarix specimens stemming from Kalurkot and Bhakkar region revealed the highest dissimilarity level with respect to the rest of the specimens.

柳是of科的重要属，以其药用价值而闻名。它的分类学很棘手，一些分子制造商可用于物种鉴定。本研究旨在评估从巴基斯坦旁遮普邦的塔尔沙漠采集的21个Ta柳标本的遗传多样性。使用了十个简单序列间重复（ISSR）和六个简单序列重复（SSR）标记。结果显示，ISSR标记产生131条带，其中116条为多态性（88.5％），平均多态信息含量（PIC）值为0.34。SSR标记产生30条扩增带，其中24条为多态性（80.28％），平均PIC值为0.35。聚类和因子分析显示，经过测试的柳杉属植物具有较高的遗传变异性生态型。差异指数表明测试样本之间的遗传变异性在0.00到0.77之间。ISSR分析表明，源自Kalurkot和Bhakkar地区的Tamarix标本相对于其余标本显示出最高的相似度。