Spent yeast cells are gained as by-products of beer and bioethanol production. Their capability as natural carrier material is underestimated. The concentrated powder form (CPF) technology is a high-pressure spray process suitable for loading a carrier material with flavours under particularly gentle process conditions, e.g. an inert gas atmosphere and low temperatures. In this study, a new process for the encapsulation of limonene, combining the gentle CPF process and emptied yeast cells as carrier material, is presented. Yeast cells are loaded with limonene and water. The water is necessary to open the pores of the cell wall and therefore to allow the limonene to enter the cell. Yeast cells with limonene contents of up to 27.4 wt-% were produced. Remarkably, the produced limonene-filled yeast cells withstand, after drying, harsh washing with hexane without losing limonene in significant amounts. SEM pictures show that the yeast cell surfaces are intact after processing.

用过的酵母细胞是啤酒和生物乙醇生产的副产品。它们作为天然载体材料的能力被低估了。浓缩粉末形式（CPF）技术是一种高压喷涂工艺，适用于在特别温和的工艺条件（例如惰性气体气氛和低温）下向载体材料中添加调味剂。在这项研究中，提出了一种将柠檬烯包封的新工艺，该工艺结合了柔和的CPF工艺和倒空的酵母细胞作为载体材料。酵母细胞充满柠檬烯和水。水是打开细胞壁孔并因此使柠檬烯进入细胞所必需的。产生了柠檬烯含量高达27.4重量％的酵母细胞。值得注意的是，干燥后产生的柠檬烯填充酵母细胞可以经受住 用己烷严格洗涤，而不会大量损失柠檬烯。SEM照片显示加工后酵母细胞表面是完整的。