Senile hypertension affects the life quality of aged population. Dietary intervention plays a pivotal role in the prevention of hypertension. There are few reports concerning the effects and mechanisms of green tea supplementation preventing age related hypertension. The current study investigated the effect and mechanism of dietary supplement of Huangshan Maofeng green tea (HSMF) on prevention of hypertension induced by deoxycorticosterone acetate (DOCA) and salt in old C57BL/6 mice. Our results showed that HSMF dose-dependently prevented the increase of systolic blood pressure and diastolic blood pressure induced by DOCA plus salt (DS) at 51-week-old mice. And HSMF significantly reduced the agonists' stimulated contraction of mesenteric arteries isolated from the old mice. The expression of vasoconstrictor genes and inflammatory cytokines in aorta were suppressed observably by HSMF supplementation compared with DS group. The protein expression of PKCα in the aorta was dose-dependently decreased by HSMF compared to DS group. The phosphorylation level of MYPT1, CPI-17and MLC₂₀ was also restrained by HSMF in the aorta. Furthermore, HSMF protected kidney by maintaining integrity of glomeruli and tubules and remarkably decreased the NGAL level in plasma. HSMF also suppressed the kidney inflammation by decreasing inflammatory cytokines expression and the macrophage infiltration. Our results proved that dietary supplement of HSMF remarkably improved the vascular functions and protected kidney injury, and thus prevented hypertension induced by DS in older C57BL/6 mice. Our data indicated that the dietary supplement of HSMF may potentially be used as a food additive for preventing hypertension for aged people.

老年高血压影响老年人的生活质量。饮食干预对于高血压的预防起着至关重要的作用。关于补充绿茶预防年龄相关性高血压的作用和机制的报道很少。本研究探讨膳食补充剂黄山毛峰绿茶（HSMF）预防老年C57BL/6小鼠醋酸脱氧皮质酮（DOCA）和盐诱导的高血压的作用及机制。我们的结果表明，HSMF 剂量依赖性地阻止 DOCA 加盐 (DS) 引起的 51 周龄小鼠收缩压和舒张压升高。 HSMF 显着降低了激动剂刺激的老年小鼠肠系膜动脉收缩。与DS组相比，补充HSMF可明显抑制主动脉中血管收缩基因和炎症细胞因子的表达。与 DS 组相比，HSMF 组主动脉 PKCα 蛋白表达呈剂量依赖性降低。主动脉中MYPT1、CPI-17和MLC ₂₀的磷酸化水平也受到HSMF的抑制。此外，HSMF 通过维持肾小球和肾小管的完整性来保护肾脏，并显着降低血浆中的 NGAL 水平。 HSMF 还通过减少炎症细胞因子表达和巨噬细胞浸润来抑制肾脏炎症。我们的结果证明，膳食补充HSMF可显着改善老年C57BL/6小鼠的血管功能并保护肾损伤，从而预防DS诱发的高血压。我们的数据表明，HSMF膳食补充剂有可能用作预防老年人高血压的食品添加剂。