Newcastle Disease Virus (NDV) is the important pathogen of Newcastle Disease (ND) attacking chicken, turkey and other birds. Therefore, the purpose of this study was to assess the effects of immune stress induced by ND vaccination and lipopolysaccharide (LPS) on growth performance, antioxidant ability, and lipid metabolism of broilers. In total, 128 one-day-old broilers were randomly assigned to the following four groups and were treated as indicated: normal control (NC); vaccinated with live LaSota ND vaccine (CV); administered ND vaccine and 0.25 mg/kg body weight (BW) LPS (L-LPS); and administered ND vaccine and 0.5 mg/kg BW LPS (H-LPS). The results demonstrated that broiler feed conversion ratio (FCR) was increased in the groups CV, L-LPS and H-LPS from d 0 to 42 days compared with the group NC. The antioxidant function of broilers was decreased as indicated by the malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) levels in the serum of the treated groups. ND vaccination combined LPS increased the concentration of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), but decreased the concentration of high-density lipoprotein cholesterol (HDLC) compared with the group NC. The reverse transcription (RT)-PCR results revealed that the mRNA expression of acetyl-CoA carboxylase gene (ACC) and 3-hydroxy-3-methylglutaryl coenzyme-A reductase (HMGR) in the liver were downregulated, whereas the mRNA expression of carnitine palmitoyltransferase-1 (CPT-1) and peroxisome proliferator-activated receptor (PPAR)-α were upregulated compared with the group NC. These results suggest that ND vaccination combined LPS reduced broiler growth performance and antioxidant ability, whereas it activated AMPK-mediated lipid metabolism.

新城疫病毒（NDV）是新城疫（ND）袭击鸡，火鸡和其他鸟类的重要病原体。因此，本研究的目的是评估ND疫苗和脂多糖（LPS）诱导的免疫应激对肉仔鸡生长性能，抗氧化能力和脂质代谢的影响。总共将128只1日龄肉鸡随机分配到以下4组中，并按指示进行处理：正常对照组（NC）; 已接种LaSota ND活疫苗（CV）；给予ND疫苗和0.25 mg / kg体重（BW）LPS（L-LPS）；并接种ND疫苗和0.5 mg / kg体重脂多糖（H-LPS）。结果表明，与NC组相比，CV，L-LPS和H-LPS组从第0天到42天增加了肉鸡饲料转化率（FCR）。如治疗组血清中的丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）水平所示，肉鸡的抗氧化功能下降。ND疫苗联合LPS可以增加总胆固醇（TC），甘油三酸酯（TG）和低密度脂蛋白胆固醇（LDL-C）的浓度，但降低高密度脂蛋白胆固醇（HDL）的浓度C）与组NC比较。逆转录（RT）-PCR结果显示，肝脏中乙酰辅酶A羧化酶基因（ACC）和3-羟基-3-甲基戊二酰辅酶A还原酶（HMGR）的mRNA表达下调，而肉碱的mRNA表达下降与NC组相比，棕榈酰转移酶-1（CPT-1）和过氧化物酶体增殖物激活受体（PPAR）-α上调。这些结果表明，ND疫苗联合LPS降低了肉鸡的生长性能和抗氧化能力，而它激活了AMPK介导的脂质代谢。