Abstract The effect of melatonin treatment on color, chlorophyll degradation and chloroplast structure in broccoli during storage was evaluated. One hundred μmmol L−1 melatonin markedly extended the shelf life of broccoli. Compared with control florets, melatonin treatment delayed the decline of chlorophyll content, and maintained more intact chloroplasts. Meanwhile, melatonin also suppressed the activities of chlorophyll catabolic enzymes such as chlorophyllase (CLH), pheophytinase (PPH), pheophorbide a oxygenase (PAO) and red chlorophyll catabolite reductase (RCCR), and down-regulated the expressions of BoNYC1, BoNOL, BoCLH, BoPPH, BoPAO, BoRCCR and BoSGR1 involved in chlorophyll catabolism. Taken together, our results indicated that melatonin could be a good candidate for delaying yellowing in broccoli during storage at 20 ℃.

中文翻译：

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褪黑激素通过调节叶绿素分解代谢和维持叶绿体超微结构延缓西兰花在储存期间变黄

摘要 研究了褪黑激素处理对西兰花贮藏过程中颜色、叶绿素降解和叶绿体结构的影响。100 μmmol L−1 褪黑激素显着​​延长了西兰花的保质期。与对照小花相比，褪黑激素处理延缓了叶绿素含量的下降，并保持了更完整的叶绿体。同时，褪黑激素还抑制叶绿素酶（CLH）、脱镁叶绿素酶（PPH）、脱镁叶绿素a加氧酶（PAO）和红色叶绿素分解代谢物还原酶（RCCR）等叶绿素分解酶的活性，下调BoNYC1、BoNOL、BoCLH的表达、BoPPH、BoPAO、BoRCCR 和 BoSGR1 参与叶绿素分解代谢。综上所述，我们的结果表明，褪黑激素可能是延迟西兰花在 20 ℃ 储存期间变黄的良好候选物。