Abstract Based on the grating structure of discarded optical disks, a fluorescent substrate with simple operation, excellent performance, and low cost were synthesized and applied to the detection of toxic molecule crystal violet (CV). The effect of the width and depth of the grating on the fluorescence signal was explored by using cleverly the natural structural differences of the three kinds of optical disks and the method of magnetron sputtering. To obtain the best substrate, two kinds of fluorescent molecules were used to screen and verify the microstructure of different morphologies. Besides, the three-dimensional finite-difference time-domain (FDTD) method is used to analyze the intensity of the local electromagnetic field and the size of the “hot spots” area of the three kinds of optimal substrates. The experimental results show that the substrates have excellent stability (reduced by 7.26% after one week) and reproducibility (RSD ≤ 4.85%), and the detection of CV molecule also shows good performance (LOD = 10−10 M). Therefore, the substrate has great potential value in biological and chemical sensing, and it can also provide a new design idea for the study of surface enhanced fluorescence (SEF) substrate.

中文翻译：

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基于三种光盘增强光栅结构的荧光研究

摘要 基于废弃光盘的光栅结构，合成了一种操作简单、性能优良、成本低廉的荧光基片，并将其应用于有毒分子结晶紫（CV）的检测。巧妙利用三种光盘的自然结构差异和磁控溅射的方法，探索了光栅的宽度和深度对荧光信号的影响。为获得最佳底物，采用两种荧光分子对不同形貌的微观结构进行筛选和验证。此外，利用三维时域有限差分（FDTD）方法分析了三种最优基板的局部电磁场强度和“热点”区域的大小。实验结果表明，该底物具有优异的稳定性（一周后降低7.26%）和重现性（RSD≤4.85%），CV分子的检测也表现出良好的性能（LOD=10-10M）。因此，该基板在生物和化学传感方面具有巨大的潜在价值，也可为表面增强荧光（SEF）基板的研究提供新的设计思路。