Background

Probiotics have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate the effect of improved formulation of chitosan-alginate microcapsules of Bifidobacterium strains on serum triglycerides, cholesterol, HDL, and LDL in mice.

Methods

Five approved probiotic strains of Bifidobacterium were tested for anti-proliferative effect and interleukin-8 induction on HT-29 cell lines. Bifidobacterium strains plus five approved Lactobacillus were encapsulated in chitosan-alginate microcapsules and tested for its survival in simulated gastrointestinal conditions. These microcapsules were administered to 4 groups of mice (including 1. Bif (Bifidobacterium strains), 2. Lac (Lactobacillus strains), 3. Bif-Lac (Bifidobacterium plus Lactobacillus strains) and 4. Control) for 8 days. At eighth day, the blood of mice were taken and serum levels of triglycerides, cholesterol, HDL, and LDL of them were determined.

Results

All of the Bifidobacterium strains significantly (P < 0.001) reduced secretion of IL-8 in HT-29 cells as well as maximum antiproliferative effects (P < 0.001). In addition, all microcapsules showed impressive survival rate in bile (>%94.1) and gastrointestinal (>%78.28) conditions (P < 0.05). Only Bif-Lac group displayed significantly lower serum cholesterol and LDL levels than control group (P < 0.05). Besides, all groups indicate statistically significant weight loss of mice during the 8 days in comparison with the control group (P < 0.05).

Conclusion

The results of this study showed that the microencapsulated probiotics with alginate and chitosan had an effective mean of delivery of viable bacterial cells and non-pharmacological interventions use to reduce serum cholesterol and LDL levels in in-vivo condition.

中文翻译：

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改良双歧杆菌壳聚糖-海藻酸钠微胶囊制剂对小鼠血脂的影响

背景

益生菌与人类消化生理学中的许多有益作用有关。这项研究的目的是评估改进的双歧杆菌菌株壳聚糖-海藻酸盐微胶囊制剂对小鼠血清甘油三酸酯，胆固醇，HDL和LDL的影响。

方法

测试了五种批准的双歧杆菌益生菌菌株对HT-29细胞系的抗增殖作用和白介素8诱导。将双歧杆菌菌株和五种批准的乳杆菌封装在壳聚糖-藻酸盐微胶囊中，并在模拟胃肠道条件下测试其存活率。将这些微胶囊施用于4组小鼠（包括1. Bif（双歧杆菌菌株），2。Lac （乳杆菌菌株），3。Bif-Lac（双歧杆菌加乳杆菌）菌株）和4.对照）8天。在第八天，取小鼠的血液，并测定它们的血清甘油三酸酯，胆固醇，HDL和LDL水平。

结果

所有双歧杆菌菌株均显着（P <0.001）减少了HT-29细胞中IL-8的分泌，并具有最大的抗增殖作用（P <0.001）。此外，所有微胶囊在胆汁（>％94.1）和胃肠道（>％78.28）条件下均显示出惊人的存活率（P <0.05）。只有Bif-Lac组的血清胆固醇和LDL水平明显低于对照组（P <0.05）。此外，与对照组相比，所有组均显示在8天中小鼠的体重减轻具有统计学意义（P <0.05）。

结论

这项研究的结果表明，具有藻酸盐和壳聚糖的微囊化益生菌可以有效地递送活细菌细胞，并且可以采用非药物干预措施降低体内条件下的血清胆固醇和LDL水平。