Objective

Candida species are the normal inhabitants of the skin and mucosa that cause a wide range of debilitating diseases in immunocompromised patients and other susceptible individuals. The present study aimed to evaluate the production of exoenzymes and the biofilm formation capacity of Candida species isolated from candidemia.

Materials and methods

In this study, a total of 100 stock Candida species isolates consist of 50 Candida albicans and 50 non-Candida albicans Candida species (24 C. glabrata, 15 C. parapsilosis, 5 C. dubliniensis, 3 C. tropicalis, 2 C. krusei and 1 C. fabianii) which previously were recovered from patients with candidemia were used. The enzymatic activity tests for hemolysin, proteinase, and phospholipase were performed by using blood Sabouraud dextrose agar, bovine serum albumin medium and egg yolk agar, respectively. Biofilm formation was determined by microplate assay method.

Result

All of the Candida albicans species could produce hemolysin. The predominant enzyme activity of species included strong and very strong levels of phospholipase, proteinase and hemolysin activity were belonged to Candida albicans isolates. There were statistically significant differences in hemolysin (P < 0.001), proteinase (P = 0.003) and phospholipase (P < 0.001) activity between two groups of albicans and non-albicans species. The biofilm formation was seen in 30 (60%) of C. albicans and 49 (98%) of non-C. albicans species. There was significant statistical differences between the two groups of isolates in biofilm formation (P < 0.001).

Conclusion

It is clear that Candida species have ability to produce several enzymes as virulence factors to contribute its pathogenicity. There were significant differences in virulence factors between the two C. albicans and non- C. albicans group. The ability for biofilm formation and producing exo-enzyme were an important virulence factors in Candida species isolates. This differences found in this report might have role in severity of disease caused by different species.

中文翻译：

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从念珠菌血症患者血流中分离出的念珠菌对毒力因子的高检测

目的

念珠菌是皮肤和粘膜的正常居民，会在免疫功能低下的患者和其他易感人群中引起各种使人衰弱的疾病。本研究旨在评估从念珠菌病分离的念珠菌物种的外切酶的产生和生物膜形成能力。

材料和方法

在这项研究中，总共有100个股票念珠菌物种分离物包括50个白色念珠菌和50个非白色念珠菌念珠菌物种（24光滑念珠菌，15个近平滑念珠菌，5个C.都柏林，3个热带念珠菌，2克柔念珠菌和以前从念珠菌血症患者中回收的1 C. fabianii）。分别通过使用血液Sabouraud葡萄糖琼脂，牛血清白蛋白培养基和蛋黄琼脂进行溶血素，蛋白酶和磷脂酶的酶活性测试。通过微板测定法确定生物膜的形成。

结果

所有白色念珠菌都可以产生溶血素。该物种的主要酶活性包括很强和很强的磷脂酶水平，蛋白酶和溶血素活性属于白色念珠菌分离物。两组白色念珠菌和非白色念珠菌之间的溶血素（P <0.001），蛋白酶（P = 0.003）和磷脂酶（P <0.001）活性有统计学差异。生物膜形成在30（60％）被认为的白色念珠菌非和49（98％）白色念珠菌物种。两组分离物在生物膜形成上有统计学差异（P <0.001）。

结论

显然，念珠菌具有产生几种酶作为致病因子的能力，从而有助于其致病性。两种C的毒力因子有显着差异。白色念珠菌和非白色念珠菌。生物膜形成和产生外切酶的能力是念珠菌物种分离物中的重要毒力因子。本报告中发现的这种差异可能与由不同物种引起的疾病严重程度有关。